Surfactant associated protein-A inhibits human lymphocyte proliferation and IL-2 production
| Autor: | James F. Lewis, Fred Possmayer, R G McFadden, Ruud A. W. Veldhuizen, Anita Caveney, Laurence J. Fraher, Paul Borron, Kevin Inchley |
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| Rok vydání: | 1996 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pulmonary Surfactant-Associated Proteins CD3 Complex Microgram Proteolipids T-Lymphocytes Clinical Biochemistry Lymphocyte Activation Peripheral blood mononuclear cell Monocytes Cell Line Pulmonary surfactant Concanavalin A Animals Humans Molecular Biology Pulmonary Surfactant-Associated Protein A Glycoproteins biology Antibodies Monoclonal Pulmonary Surfactants Cell Biology Pneumonia Molecular biology In vitro Recombinant Proteins Biochemistry biology.protein Interleukin-2 Cattle Antibody Protein A Cell Division |
| Zdroj: | ResearcherID |
| ISSN: | 1044-1549 |
| Popis: | The hyporesponsive state of lung-derived mononuclear leukocytes has been, in part, attributed to the effects of the lipid rather than the protein components of pulmonary surfactant. In the present study, however, the results suggest that purified preparations of pulmonary surfactant-associated protein A (SP-A) suppress both phytohemagglutinin (PHA, 1 microgram/ml)- and anti-CD-3 (1 to 10 ng/ml) activated proliferation of human peripheral blood and tonsillar mononuclear cells in a dose-dependent manner at concentrations as low as 50 pM (6.25 micrograms/ml) when added at the initiation of cultures. Addition of SP-A to PHA-stimulated peripheral blood mononuclear cells (PBMC) as late as 24 to 36 h after PHA was also capable of suppressing [3H]thymidine incorporation measured at 72 h. In contrast, concanavalin A (Con A; 2 micrograms/ml)-stimulated PBMC proliferation was slightly augmented by the addition of SP-A. Analysis of the supernatants of PHA-stimulated cultures treated with SP-A revealed that accompanying the inhibition of proliferation was a corresponding decline in measurable interleukin-2 (IL-2) concentrations, from 154 pg/ml for the PHA-treated cells to 57.8, 28.4, 5.2, and less than 2 pg/ml of IL-2 when SP-A was added at 6.25, 12.5, 25, and 50 micrograms/ml, respectively. We suggest that the action of SP-A on PHA-stimulated human PBMC may involve the blocking of a costimulatory signal crucial for in vitro T-cell activation. |
| Databáze: | OpenAIRE |
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