Bacterial And Archeal Dynamics Of A Labscale Hybrid Gas Fermentation Bioreactor Fed With Co2 And H-2
| Autor: | Nuri Azbar, Burçin Karabey, Sidika Tugce Daglioglu, Guven Ozdemir |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
biology Chemistry 020209 energy Methanobacteriales 02 engineering and technology General Medicine Methanosaeta concilii 010501 environmental sciences biology.organism_classification 01 natural sciences Methanoculleus Microbial population biology DGGE (denaturing gradient gel electrophoresis) quantitative PCR 0202 electrical engineering electronic engineering information engineering Methanosarcinales Bioreactor hydrogenotrophic methanogen Methanomicrobiales Fermentation CO2 Food science 0105 earth and related environmental sciences Biomethane |
| Popis: | EgeUn### 16s rDNA-based methods were used in order to identify the dynamics of microbial profiles in a HYBRID gas fermentation bio-methanization reactor. The effects of various H-2 and CO2 ratios on microbial community were investigated. The HYBRID gas fermentation reactor was composed of granular anaerobic seed and the system fed with only H-2 and CO2 gases. No additional organic material and trace element was fed during the throughout the experiments; thus, the microbial diversity was directly related to production of methane. The dynamics of the microbial communities were investigated with DGGE and real-time PCR analysis. The results showed that Methanobacteriales members were more dominated than Methanosarcinales and Methanomicrobiales members in the system. DGGE results indicated that Methanosaeta concilii, Methanoculleus sp., Methanosphaerula palustris, Methanofollis formosanus, Methanolinea sp., and Methanobacterium palustre were the most prominent methanogens depending on different H-2/CO2 ratios. DGGE profiles suggested that hydrogenotrophic and acetoclastic species were responsible for the production of methane. The survival of syntrophic bacteria and acetoclastic methanogens was attributed to their utilization of organic materials provided by lysis. To the best of our knowledge, this is the first microbial profile detection study in a hybrid bioreactor system operated with only pure hydrogen and carbon dioxide. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115Y455]; Ege University Scientific Research FundEge University [16/EBILTEM/003] The authors thank Scientific and Technological Research Council of Turkey (TUBITAK) under the grant no. 115Y455 for the financial support of this study. The authors also thank Ege University Scientific Research Fund under the grant no. 16/EBILTEM/003 for the financial support of this study. |
| Databáze: | OpenAIRE |
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