Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12
| Autor: | R A Roehl, R T Vinopal |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Mannose
Locus (genetics) Biology medicine.disease_cause Microbiology chemistry.chemical_compound Glucosamine medicine Escherichia coli Anaerobiosis Phosphoenolpyruvate Sugar Phosphotransferase System Molecular Biology chemistry.chemical_classification Structural gene Chromosome Mapping Biological Transport PEP group translocation Chromosomes Bacterial Molecular biology Enzyme Glucose chemistry Biochemistry Genes Mutation Phosphoenolpyruvate carboxykinase Research Article |
| Zdroj: | Journal of bacteriology. 142(1) |
| ISSN: | 0021-9193 |
| Popis: | Most strains of Escherichia coli K-12 are unable to use the enzyme IIA/IIB (enzyme IIMan) complex of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in anaerobic growth and therefore cannot utilize glucosamine anaerobically. Introduction into these strains of a ptsG mutation, which eliminates activity of the enzyme IIIGlc/IIB' complex of the PTS, resulted in inability to grow anaerobically on glucose and mannose. Derivative strains able to grow anaerobically on glucosamine had mutations at a locus close to man, the gene coding for phosphomannose isomerase, and had higher enzyme IIA/IIB activities during anaerobic growth than did the parental strain. These results establish a locus affecting function of enzyme IIA/IIB that maps distant from ptsM, the probable structural gene for enzyme IIB. |
| Databáze: | OpenAIRE |
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