β-Adrenergic-mediated vasodilation in young men and women: cyclooxygenase restrains nitric oxide synthase
| Autor: | Joshua J. Sebranek, Jacqueline K. Limberg, Marlowe W. Eldridge, William G. Schrage, J. Mikhail Kellawan, Garrett L. Peltonen, Rebecca E. Johansson, John W. Harrell |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Agonist medicine.medical_specialty Mean arterial pressure Physiology medicine.drug_class Prostaglandin Vasodilation 030204 cardiovascular system & hematology Microcirculation 03 medical and health sciences chemistry.chemical_compound Integrative Cardiovascular Physiology and Pathophysiology Sex Factors 0302 clinical medicine Physiology (medical) Internal medicine medicine Humans Infusions Intra-Arterial Cyclooxygenase Inhibitors Enzyme Inhibitors omega-N-Methylarginine biology business.industry Isoproterenol Ultrasonography Doppler Adrenergic beta-Agonists Nitric oxide synthase Forearm Endocrinology chemistry Prostaglandin-Endoperoxide Synthases biology.protein Omega-N-Methylarginine Female Cyclooxygenase Nitric Oxide Synthase Cardiology and Cardiovascular Medicine business Ketorolac 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 310:H756-H764 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00886.2015 |
| Popis: | We tested the hypothesis that women exhibit greater vasodilator responses to β-adrenoceptor stimulation compared with men. We further hypothesized women exhibit a greater contribution of nitric oxide synthase and cyclooxygenase to β-adrenergic-mediated vasodilation compared with men. Forearm blood flow (Doppler ultrasound) was measured in young men ( n = 29, 26 ± 1 yr) and women ( n = 33, 25 ± 1 yr) during intra-arterial infusion of isoproterenol (β-adrenergic agonist). In subset of subjects, isoproterenol responses were examined before and after local inhibition of nitric oxide synthase [ NG-monomethyl-l-arginine (l-NMMA); 6 male/10 female] and/or cyclooxygenase (ketorolac; 5 male/5 female). Vascular conductance (blood flow ÷ mean arterial pressure) was calculated to assess vasodilation. Vascular conductance increased with isoproterenol infusion ( P < 0.01), and this effect was not different between men and women ( P = 0.41). l-NMMA infusion had no effect on isoproterenol-mediated dilation in men ( P > 0.99) or women ( P = 0.21). In contrast, ketorolac infusion markedly increased isoproterenol-mediated responses in both men ( P < 0.01) and women ( P = 0.04) and this rise was lost with subsequent l-NMMA infusion (men, P < 0.01; women, P < 0.05). β-Adrenergic vasodilation is not different between men and women and sex differences in the independent contribution of nitric oxide synthase and cyclooxygenase to β-mediated vasodilation are not present. However, these data are the first to demonstrate β-adrenoceptor activation of cyclooxygenase suppresses nitric oxide synthase signaling in human forearm microcirculation and may have important implications for neurovascular control in both health and disease. |
| Databáze: | OpenAIRE |
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