Regulation of microtubule-associated motors drives intermediate filament network polarization
| Autor: | Cécile Leduc, Sandrine Etienne-Manneville |
|---|---|
| Přispěvatelé: | Polarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Institut National du Cancer, the Association pour la Recherche Contre le Cancer, the Ligue Contre le Cancer, the Centre National de la Recherche Scientifique, the Institut Pasteur, and the French National Research Agency (ANR-16-CE13-0019)., We thank Mathieu Piel, Arnaud Echard, Danielle Pham-Dinh, and Carine Rossé for reagents and equipment, Jean-Baptiste Manneville and the Etienne-Manneville group for continuous support and careful reading of the manuscript, and Stéphanie Portet and Andrea Parmeggiani and his group for stimulating discussions. We gratefully acknowledge Jean-Yves Tinevez, Audrey Salles, and the Imagopole of Institut Pasteur (Paris, France) as well as the France–BioImaging infrastructure network supported by the French National Research Agency (ANR-10–INSB–04, Investments for the Future), and the Région Ile-de-France (program Domaine d’Intérêt Majeur-Malinf) for the use of the Elyra microscope., ANR-16-CE13-0019,SiFi2Net,Filaments intermédiaires: du filament unique au réseau(2016), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
MESH: Signal Transduction
0301 basic medicine Time Factors Intermediate Filaments CDC42 Cell morphology Microtubules Nestin Cell Movement Cell polarity MESH: Glial Fibrillary Acidic Protein MESH: Animals Intermediate filament cdc42 GTP-Binding Protein MESH: Cell Movement Research Articles Cytoskeleton Protein Kinase C Microscopy Video Glial fibrillary acidic protein biology MESH: Microtubules MESH: Nestin Optical Imaging Cell Polarity Cell biology MESH: Intermediate Filaments MESH: Neuroglia RNA Interference MESH: Cell Polarity MESH: Dyneins Neuroglia Signal Transduction MESH: Cell Line Tumor MESH: Rats MESH: RNA Interference [SDV.BC]Life Sciences [q-bio]/Cellular Biology macromolecular substances Editorials: Cell Cycle Features MESH: Actins Transfection Article 03 medical and health sciences Microtubule Cell Line Tumor Glial Fibrillary Acidic Protein Animals Humans Vimentin Actin MESH: Optical Imaging Wound Healing MESH: cdc42 GTP-Binding Protein MESH: Humans MESH: Transfection MESH: Time Factors Dyneins Cell Biology MESH: Protein Kinase C Actins Rats MESH: Microscopy Video MESH: Astrocytes MESH: Wound Healing 030104 developmental biology Cytoplasm Astrocytes biology.protein MESH: Vimentin [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
| Zdroj: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2017, 216 (6), pp.1689-1703. ⟨10.1083/jcb.201607045⟩ Journal of Cell Biology, 2017, 216 (6), pp.1689-1703. ⟨10.1083/jcb.201607045⟩ |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Intermediate filaments (IFs) participate in directed cell migration, but how the IF network becomes polarized in motile cells is unclear. Leduc and Etienne-Manneville show that the turnover of IF mainly relies on actin-driven retrograde flow and microtubule-driven anterograde and retrograde transport. During cell migration, Cdc42-mediated polarity signaling inhibits dynein-dependent transport to promote the polarization of the IF network. Intermediate filaments (IFs) are key players in the control of cell morphology and structure as well as in active processes such as cell polarization, migration, and mechanoresponses. However, the regulatory mechanisms controlling IF dynamics and organization in motile cells are still poorly understood. In this study, we investigate the mechanisms leading to the polarized rearrangement of the IF network along the polarity axis. Using photobleaching and photoconversion experiments in glial cells expressing vimentin, glial fibrillary acidic protein, and nestin, we show that the distribution of cytoplasmic IFs results from a continuous turnover based on the cooperation of an actin-dependent retrograde flow and anterograde and retrograde microtubule-dependent transports. During wound-induced astrocyte polarization, IF transport becomes directionally biased from the cell center toward the cell front. Such asymmetry in the transport is mainly caused by a Cdc42- and atypical PKC–dependent inhibition of dynein-dependent retrograde transport. Our results show how polarity signaling can affect the dynamic turnover of the IF network to promote the polarization of the network itself. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|