Nuclear body movement is determined by chromatin accessibility and dynamics
| Autor: | Peter Lichter, Sabine M. Görisch, Carina Ittrich, Christian P. Bacher, Malte Wachsmuth, Karsten Rippe |
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| Rok vydání: | 2004 |
| Předmět: |
Coiled Bodies
Promyelocytic Leukemia Protein Biology Promyelocytic leukemia protein Adenosine Triphosphate Organelle Transcriptional regulation medicine Humans Nuclear protein Transcription factor Cell Nucleus Genetics Multidisciplinary Tumor Suppressor Proteins Nuclear Proteins Biological Sciences Chromatin Neoplasm Proteins Cell biology Cell nucleus medicine.anatomical_structure Cajal body biology.protein HeLa Cells Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 101:13221-13226 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0402958101 |
| Popis: | Promyelocytic leukemia (PML) and Cajal bodies are mobile subnuclear organelles, which are involved in activities like RNA processing, transcriptional regulation, and antiviral defense. A key parameter in understanding their biological functions is their mobility. The diffusion properties of PML and Cajal bodies were compared with a biochemically inactive body formed by aggregates of murine Mx1 by using single-particle tracking methods. The artificial Mx1-yellow fluorescent protein body showed a very similar mobility compared with PML and Cajal bodies. The data are described quantitatively by a mechanism of nuclear body movement consisting of two components: diffusion of the body within a chromatin corral and its translocation resulting from chromatin diffusion. This finding suggests that the body mobility reflects the dynamics and accessibility of the chromatin environment, which might target bodies to specific nuclear subcompartments where they exert their biological function. |
| Databáze: | OpenAIRE |
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