Serotonin3 receptor stimulation in the nucleus tractus solitarii activates non-catecholaminergic neurons in the rat ventrolateral medulla

Autor: Raul Laguzzi, Leni G.H. Bonagamba, J. C. Callera, Anne Nosjean, Michel Hamon, Benedito H. Machado
Přispěvatelé: Neuropsychopharmacologie moléculaire, cellulaire et fonctionnelle, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
Rok vydání: 2002
Předmět:
Male
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Biguanides
MESH: Rats
Sprague-Dawley

Urethane
Rats
Sprague-Dawley

Catecholamines
0302 clinical medicine
MESH: Animals
MESH: Tyrosine 3-Monooxygenase
Horseradish Peroxidase
Catecholaminergic
Medulla Oblongata
0303 health sciences
MESH: Solitary Nucleus
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Proto-Oncogene Proteins c-fos
Chemistry
General Neuroscience
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Rostral ventrolateral medulla
MESH: Gene Expression Regulation
Immunohistochemistry
MESH: Serotonin Receptor Agonists
Serotonin Receptor Agonists
Chloralose
MESH: Chloralose
Medulla oblongata
Brainstem
Proto-Oncogene Proteins c-fos
MESH: Receptors
Serotonin

medicine.medical_specialty
MESH: Rats
Tyrosine 3-Monooxygenase
MESH: Receptors
Serotonin
5-HT3

03 medical and health sciences
MESH: Urethane
MESH: Medulla Oblongata
Internal medicine
MESH: Catecholamines
Solitary Nucleus
medicine
Animals
MESH: Horseradish Peroxidase
Medulla
030304 developmental biology
Tyrosine hydroxylase
Solitary nucleus
MESH: Immunohistochemistry
MESH: Biguanides
MESH: Male
Rats
Endocrinology
Gene Expression Regulation
Receptors
Serotonin

Catecholaminergic cell groups
Receptors
Serotonin
5-HT3

030217 neurology & neurosurgery
Zdroj: Neuroscience
Neuroscience, Elsevier-International Brain Research Organization, 2002, 112 (4), pp.935-949. ⟨10.1016/s0306-4522(02)00057-x⟩
Neuroscience, 2002, 112 (4), pp.935-949. ⟨10.1016/s0306-4522(02)00057-x⟩
ISSN: 0306-4522
1873-7544
DOI: 10.1016/s0306-4522(02)00057-x
Popis: International audience; The present study was performed to determine whether or not the increased arterial pressure triggered by 5-HT(3) receptor stimulation in the nucleus tractus solitarii and underlain by a sympathoexcitation is associated with the activation of ventromedullary cells known to be involved in vascular regulation, i.e. the C1 and A1 catecholaminergic cells. For this purpose, double immunohistochemical labeling for tyrosine hydroxylase and c-fos protein was performed all along the ventrolateral medulla after microinjection of 1-(m-chlorophenyl)-biguanide, a selective and potent 5-HT(3) receptor agonist, into the nucleus tractus solitarii of alpha-chloralose/urethane-anaesthetized rats. This treatment produced a significant elevation of arterial pressure ( approximately +35 mm Hg). Concomitantly, a significant increase in the number of c-fos expressing neurons was observed in the rostral ventrolateral medulla (+63%), in particular in its most anterior part (+78%), and in the medullary region surrounding the caudal part of the facial nucleus (+91%). Retrograde labeling with gold-horseradish peroxidase complex showed that at least some of these activated c-fos expressing cells project to the spinal cord. However, the number of double-stained neurons, i.e. c-fos and tyrosine hydroxylase positive neurons, did not increase at any level of the ventrolateral medulla. In contrast, under the same alpha-chloralose/urethane anesthesia, systemic infusion of sodium nitroprusside appeared to produce a hypotension and a marked increase in the density of such double c-fos and tyrosine hydroxylase expressing cells in the rostral ventrolateral medulla and the caudal medullary region surrounding the caudal part of the facial nucleus. These data indicate that medullary catecholaminergic C1 and A1 neurons are not involved in the pressor effect elicited by 5-HT(3) receptor stimulation in the nucleus tractus solitarii. However, this 5-HT(3) receptor-mediated effect is clearly associated with the excitation of (non-catecholaminergic) neurons within the pressor region of the ventral medulla.
Databáze: OpenAIRE
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