Vaccination with a Brucella ghost developed through a double inactivation strategy provides protection in Guinea pigs and cattle

Autor:	Chuanyu He, Jianghua Yang, Hailong Zhao, Mengzhi Liu, Dongling Wu, Baoshan Liu, Sun He, Zeliang Chen
Rok vydání:	2021
Předmět:	Mice Inbred BALB C Guinea Pigs Vaccination Brucella Vaccine Brucella abortus Hydrogen Peroxide Microbiology Antibodies Bacterial Brucellosis Mice Infectious Diseases Brucella melitensis Animals Cattle
Zdroj:	Microbial pathogenesis. 162
ISSN:	1096-1208
Popis:	Vaccination can prevent and control animal brucellosis. Currently, live attenuated vaccines are extensively used to prevent Brucella infection. However, traditional vaccines such as live attenuated vaccines are associated with biological safety risks for both humans and animals. The bacterial ghost (BG) is a new form of vaccine with great prospects. However, bacterial cells cannot be completely inactivated by biological lysis, conferring a safety risk associated with the vaccine. In this study, we developed a Brucella abortus A19 bacterial ghost (A19BG) through a double inactivation strategy with sequential biological lysis and hydrogen peroxide treatment. This strategy resulted in 100% inactivation of Brucella, such that viable bacterial cells were not detected even at an ultrahigh concentration of 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da863dbb2bcb3f5d5432203167a0339c https://pubmed.ncbi.nlm.nih.gov/34919994 Zobrazit plný text záznamu Full Text from ScienceDirect