Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males
Autor: | Raushan K. Singh, Shivani Chhillar, Tushar Kumar Mohanty, Sudhanshu Bala Nayak, N.M. Attupuram, S. K. Rajak, R. K. Baithalu, L. Sreela, Arumugam Kumaresan, Savita Yadav, U.K. Tripathi |
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Rok vydání: | 2016 |
Předmět: |
Anti-Mullerian Hormone
Male endocrine system medicine.medical_specialty Aging endocrine system diseases Transcription Genetic Enzyme-Linked Immunosorbent Assay Crossbreed 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Food Animals Internal medicine Blood plasma medicine Animals Small Animals Regulation of gene expression 030219 obstetrics & reproductive medicine Sertoli Cells biology urogenital system Equine 0402 animal and dairy science Anti-Müllerian hormone 04 agricultural and veterinary sciences Zebu Sertoli cell 040201 dairy & animal science Endocrinology medicine.anatomical_structure Gene Expression Regulation biology.protein Animal Science and Zoology Cattle hormones hormone substitutes and hormone antagonists Hormone |
Zdroj: | Theriogenology. 89 |
ISSN: | 1879-3231 |
Popis: | Age-related changes in peripheral anti-Mullerian hormone (AMH) concentrations and transcriptional abundance of AMH gene in testicular tissue were studied in crossbred (Holstein Friesian × Tharparkar) and Zebu (Tharparkar) males. In both the breeds, basal AMH concentrations were estimated using ELISA method in blood plasma obtained from six males each at 1, 6, 12, 18, and 24 months age. After blood collection at respective ages, all the males were castrated and expression and immunolocalization of AMH was performed in the testicular tissue. The concentration of AMH in blood plasma was found to be highest at 1 month of age in both crossbred and Zebu males, which subsequently decreased with advancing age. Significantly (P |
Databáze: | OpenAIRE |
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