Heavy Ion Beam Irradiation Regulates the mRNA Expression in Megakaryocytopoiesis from Human Hematopoietic Stem/Progenitor Cells
| Autor: | Ken Itoh, Kenji Takahashi, Ikuo Kashiwakura, Hironori Yoshino, Satoru Monzen, Atsushi Maruyama, Kiyomi Kasai-Eguchi, Yoshinao Abe |
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| Rok vydání: | 2009 |
| Předmět: |
Radiation
Health Toxicology and Mutagenesis CD34 Biology Hematopoietic Stem Cells Thrombopoiesis Cell biology Heme oxygenase Haematopoiesis Gene Expression Regulation Gene expression Immunology Humans Erythropoiesis Heavy Ions Radiology Nuclear Medicine and imaging RNA Messenger Progenitor cell Stem cell Cells Cultured Megakaryocytopoiesis |
| Zdroj: | Journal of Radiation Research. 50:477-486 |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1269/jrr.09058 |
| Popis: | Heavy ion beams are a high-LET radiation that has greater biological effect than electron beams or X-rays. However, little is known about the effect of heavy ion beams on the proliferation and differentiation of human hematopoietic stem/progenitor cells (HSPCs). The present study examined the effect of heavy ion beams on gene expression in human HSPCs, especially during early stage of megakaryocytopoiesis. Human CD34+ cells were exposed to monoenergetic carbon-ion beams (290 MeV/nucleon, LET = 50 KeV/m) that were generated by an accelerator (Heavy Ion Medical Accelerator in Chiba). The expression of various genes related to early hematopoiesis, megakaryocytopoiesis/erythropoiesis, cytokine receptors and oxidative stress were analyzed by real-time RT-PCR. Friend leukemia virus integration 1, an early hematopoiesis-related gene, showed significantly higher mRNA expression than the control at 6 hr after irradiation. In contrast, no significant differences were observed in almost all of the other early hematopoiesis-related genes, cytokine receptor-coded genes and megakaryocytopoiesis/erythropoiesis-differentiation pathway-related genes, respectively. An analysis of the response of the genes to oxidative stress revealed the expression of heme oxygenase 1 to show a 1.5-fold and 11.9-fold increase from the day 0 control at 24 hr after 0.5 Gy and 2 Gy irradiation, respectively. Similarly, the NAD(P)H dehydrogenase-quinone 1 expression also showed a 22.0-fold and a 21.8-fold increase at 6 hr in comparison to the initial control. These results showed that the heavy ion beams affect megakaryocytopoiesis/ erythropoiesis differentiation of human HSPCs on the gene expression level. |
| Databáze: | OpenAIRE |
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