Transcriptome, cytological and biochemical analysis of cytoplasmic male sterility and maintainer line in CMS-D8 cotton
| Autor: | Yuanlong Wu, Chaozhu Xing, Meng Zhang, Jianyong Wu, Shuangxia Jin, Li Yang, Jinfa Zhang, James McD. Stewart |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell signaling RNA-Seq Plant Science Mitochondrion Biology 01 natural sciences Transcriptome 03 medical and health sciences Microscopy Electron Transmission Genetics health care economics and organizations chemistry.chemical_classification Gossypium Tapetum Reactive oxygen species Gene Expression Profiling Cytoplasmic male sterility RNA General Medicine Cell biology 030104 developmental biology chemistry Infertility Pollen Reactive Oxygen Species Oxidation-Reduction Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Molecular Biology. 97:537-551 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Key message This research based on RNA-seq, biochemical, and cytological analyses sheds that ROS may serve as important signaling molecules of cytoplasmic male sterility in CMS-D8 cotton. To understand the mechanism of cytoplasmic male sterility in cotton (Gossypium hirsutum), transcriptomic, cytological, and biochemical analysis were performed between the cytoplasmic male sterility CMS-D8 line, Zhong41A, and its maintainer line Zhong41B. A total of 2335 differentially expressed genes (DEGs) were identified in the CMS line at three different stages of anther development. Bioinformatics analysis of these DEGs indicated their relationship to reactive oxygen species (ROS) homeostasis, including reduction-oxidation reactions and the metabolism of glutathione and ascorbate. At the same time, DEGs associated with tapetum development, especially the transition to secretory tapetum, were down-regulated in the CMS line. Biochemical analysis indicated that the ability of the CMS line to eliminate ROS was decreased, which led to the rapid release of H2O2. Cytological analysis revealed that the most crucial defect in the CMS line was the abnormal tapetum. All these results are consistent with the RNA sequencing data. On the basis of our findings, we propose that ROS act as signal molecules, which are released from mitochondria and transferred to the nucleus, triggering the formation of abnormal tapetum. |
| Databáze: | OpenAIRE |
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