FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate

Autor: Orlando Ruiz, Juan Pablo Arias, Dary Mendoza, Olmedo Cuaspud, Mario Lobo Arias
Rok vydání: 2020
Předmět:
Plant cell culture
0106 biological sciences
SH
Schenk and Hildebrandt
Metabolite
Flavonoid
FW
fresh weight
OPLS-DA
orthogonal partial least square-discriminant analysis
01 natural sciences
Applied Microbiology and Biotechnology
DW
dry weight
chemistry.chemical_compound
GAE
gallic acid equivalents
MeJA
Methyl jasmonate
COW
Correlation Optimized Warping
RP-HPLC
reversed phase-high performance liquid chromatography
chemistry.chemical_classification
0303 health sciences
Thevetia
Methyl jasmonate
biology
MVA
multivariate analysis
PLS
partial least square-discriminant analysis
DV2
second derivatives
Elicitor
DV1
first derivatives
SNV
Standard Normal Variate
PC
phenolic compounds
FT-NIR
Research Article
Thevetia peruviana
Biotechnology
DHQ
dihydroquercetin
lcsh:Biotechnology
KT
Kinetin
SG
Savitzky Golay
03 medical and health sciences
Biosynthesis
lcsh:TP248.13-248.65
010608 biotechnology
2
4-D
2
4-dichlorophenoxy acetic acid
SA
salicylic acid
ComputingMethodologies_COMPUTERGRAPHICS
030304 developmental biology
PCA
principal component analysis
Chromatography
Plant cell
biology.organism_classification
CGA
chlorogenic acid
Multivariate analysis
chemistry
FT-NIR
fourier transform near-infrared spectroscopy
RP-HPLC
Salicylic acid
Zdroj: Biotechnology Reports
Biotechnology Reports, Vol 27, Iss, Pp e00519-(2020)
ISSN: 2215-017X
Popis: Graphical abstract
Highlights • Near infrared spectroscopy was used for the detection of phenolic content in plant cell cultures. • Multivariate analysis applied to HPLC data was satisfactory to determine changes in the phenolic profile. • Dihydroquercetin increased significantly in T. peruviana cultures treated with SA/MeJA. • Chlorogenate and dihydroquercetin are possible biomarkers of the MeJA effects in T. peruviana.
Plant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spectroscopy and Reversed-phase high-performance liquid chromatography (RP-HPLC) combined with Multivariate analysis (MVA) were used to detect significant differences in the PC production in cultures treated with two elicitors. The results suggest that the FT-NIR-MVA is useful for discriminating samples according to the treatment, showed significant influence of the PC signal. RP-HPLC-MVA showed that the elicitor effect occurs at 72 h post-elicitation. Detection of dihydroquercetin (maximum concentration = 12.59 mg/L), a flavonoid with anti-cancer properties, is highlighted. Future studies will be aimed at scaling this culture to increase the productivity of dihydroquercetin.
Databáze: OpenAIRE
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