Multiple optical stimulation to neuron using Si opto-neural probe with multiple optical waveguides and metal-cover for optogenetics
| Autor: | Hajime Mushiake, Hisashi Kino, H. Yao, T. Harashima, Toshinobu Kuki, S. Kanno, Sang Hern Lee, Tetsu Tanaka |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Silicon
Materials science Optical fiber genetic structures Light chemistry.chemical_element Action Potentials Nanotechnology Local field potential Optogenetics law.invention Electricity law Electric Impedance Animals Stimulated emission Electrodes Leakage (electronics) Neurons business.industry Brain Electrochemical Techniques Neurophysiology Current source eye diseases chemistry Metals Optoelectronics Microtechnology sense organs Rats Transgenic business Photic Stimulation |
| Zdroj: | EMBC |
| ISSN: | 2694-0604 |
| Popis: | We have developed a Si opt-neural probe with multiple waveguides and metal cover for highly accurate optical stimulation. This neural probe had 16 recording sites, three optical waveguides, and metal cover for suppressing light leakage. We evaluated electrochemical properties of the recording sites, and confirmed that the neural probe had suitable characteristics for neural recording. We also demonstrated the optical stimulation to the neurons expressing ChR2 using our probe. As a result, we succeeded multisite optical stimulation, and observed that no light leakage from the optical waveguides because of the metal cover. From in vivo experiments, we successfully recorded optically modulated local field potential using the fabricated Si neural probe with optical waveguides. Moreover, we applied current source density analysis to the recorded LFPs. As a result, we confirmed that light induced membrane current sink in locally stimulated area. Our Si opto-neural probe with multiple optical waveguides and metal-cover is one of the most versatile tools for optogenetics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|