Transcriptome-Wide Identification of Reference Genes for Expression Analysis of Soybean Responses to Drought Stress along the Day
| Autor: | Mayla Daiane Correa Molinari, Frank G. Harmon, Rafaela Ribeiro Reis, Renata Fuganti-Pagliarini, José Renato Bouças Farias, Juliana Marcolino-Gomes, Alexandre Lima Nepomuceno, Fabiana Aparecida Rodrigues, Thiago Jonas Nakayama, Hugo Bruno Correa Molinari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Candidate gene
Circadian clock lcsh:Medicine Biology Genes Plant Polymerase Chain Reaction Transcriptome Gene Expression Regulation Plant Reference genes Botany lcsh:Science Gene Library Genetics Regulation of gene expression Multidisciplinary Dehydration Gene Expression Profiling lcsh:R fungi food and beverages Housekeeping gene Gene expression profiling RNA Plant lcsh:Q Soybeans DNA microarray Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 9, p e0139051 (2015) |
| ISSN: | 1932-6203 |
| Popis: | The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptome-wide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|