Sex control by Zfy siRNA in the dairy cattle
| Autor: | Xuhai Wang, Chaocheng Li, Rui-wen Ying, Bin Jia, Yongsheng Zhang, Xiang-zu Wang, Jifeng Xi |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Sex Determination Analysis Offspring medicine.medical_treatment Kruppel-Like Transcription Factors Semen Biology Insemination Y chromosome Animals Genetically Modified Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Food Animals Y Chromosome medicine Animals RNA Small Interfering Cells Cultured Insemination Artificial Dairy cattle Sperm motility 030219 obstetrics & reproductive medicine Artificial insemination 0402 animal and dairy science Gene Expression Regulation Developmental Zinc Fingers 04 agricultural and veterinary sciences General Medicine Sex Determination Processes 040201 dairy & animal science Dairying Cattle Female Animal Science and Zoology Spermatogenesis |
| Zdroj: | Animal Reproduction Science. 200:1-6 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2018.05.015 |
| Popis: | Zinc-finger Y is located in the short arm of the Y-chromosome and is a highly conserved gene that plays an important role in spermatogenesis. The objective of this study was to investigate the influence of silencing the Zfy gene during spermatogenesis on Y-sperm formation and offspring sex determination in Bos taurus cattle. Three recombinant expression vectors pLL3.7/a, pLL3.7/b and pLL3.7/c were evaluated and only pLL3.7/a effectively silenced the Zfy gene. The pLL3.7/a recombinant expression vector was injected into bull testes, using three injections. Semen was collected and preserved by extending and freezing. The frozen semen was subsequently used in artificial insemination of cows during a breeding season in accordance with the production plan on the farm where the experiment was conducted. Results showed that, after exposure to pLL3.7/a, sperm motility decreased (P 0.01), but the sperm density was similar (p 0.05) to the non-treated control semen. Injection of pLL3.7/a resulted in 72.0% female offspring, and was greater than the 49.4% female calves in the control (P ＜ 0.01), Results from this research suggests that the Zfy gene plays a role in the process of Y-sperm formation, and Zfy siRNA is a potential useful approach to control sex of offspring in cattle. |
| Databáze: | OpenAIRE |
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