Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling
| Autor: | Yizhong Liu, Eunsoo Yoo, Chendong Han, Gretchen J. Mahler, Amber L. Doiron |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Myosin light-chain kinase Chemistry Materials Science (miscellaneous) Public Health Environmental and Occupational Health Actin remodeling 02 engineering and technology Raft 021001 nanoscience & nanotechnology Endocytosis Calcium in biology Article 03 medical and health sciences 030104 developmental biology Caveolae Biophysics Viability assay 0210 nano-technology Safety Risk Reliability and Quality Safety Research Calcium signaling |
| Zdroj: | NanoImpact. 11 |
| ISSN: | 2452-0748 |
| Popis: | The rapid development of modern nanotechnology has resulted in nanomaterial being use in nearly all applications of life, raising the potential risk of nanomaterial exposure alongside the need to design safe and effective materials. Previous work has demonstrated a specific effect of gold nanoparticles (GNPs) of approximately 20 nm on endothelial barrier function in vitro. To expand our understanding of this size-specific effect, titanium dioxide, silicon dioxide, and polystyrene nanoparticles (NPs) in this similar size range were studied. All tested nanoparticles were found to have minimal effects on cell viability, but exhibited a significant detrimental effect on endothelial barrier function. Nanoparticles in the size range of 20 to 30 nm were internalized by endothelial cells through caveolae/raft-mediated endocytosis, causing intracellular calcium elevation by approximately 30% at 2 h after administration, and triggering myosin light chain kinase (MLCK)-regulated actomyosin contraction. These effects culminated in an increase in endothelial monolayer permeability across all particle types within the 20–30 nm range. This nanoparticle exposure-induced endothelial barrier dysfunction may provide valuable information for designing safer nanomaterials or potential applications of this nanoparticle exposure-induced permeability effect in biomedicine. |
| Databáze: | OpenAIRE |
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