Enhancing the specificity of polymerase chain reaction by graphene oxide through surface modification: zwitterionic polymer is superior to other polymers with different charges
| Autor: | Zhisen Zhang, Jian Weng, Liping Sun, Xiong Yunjing, Yong Zhong, Lihong Huang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Polymers Surface Properties Polyacrylamide Biophysics Acrylic Resins Pharmaceutical Science Bioengineering 02 engineering and technology Polyethylene glycol DNA Mitochondrial Polymerase Chain Reaction Sensitivity and Specificity Polyethylene Glycols Biomaterials 03 medical and health sciences chemistry.chemical_compound Betaine charge International Journal of Nanomedicine Drug Discovery Organic chemistry Humans Pfu DNA polymerase Surface charge Original Research chemistry.chemical_classification Electrophoresis Agar Gel Organic Chemistry Polyacrylic acid Oxides General Medicine Polymer DNA 021001 nanoscience & nanotechnology Combinatorial chemistry Molecular Docking Simulation 030104 developmental biology chemistry pSB Solvents Surface modification Graphite 0210 nano-technology PCR additive |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Yong Zhong,1,* Lihong Huang,2,* Zhisen Zhang,3 Yunjing Xiong,1 Liping Sun,1 Jian Weng1 1Department of Biomaterials, College of Materials, 2State Key Laboratory of Cellular Stress Biology, School of Life Sciences, 3Department of Physics, Research Institute for Biomimetics and Soft Matter, Xiamen University, Xiamen, People’s Republic of China *These authors contributed equally to this work Abstract: Graphene oxides (GOs) with different surface characteristics, such as size, reduction degree and charge, are prepared, and their effects on the specificity of polymerase chain reaction (PCR) are investigated. In this study, we demonstrate that GO with a large size and high reduction degree is superior to small and nonreduced GO in enhancing the specificity of PCR. Negatively charged polyacrylic acid (PAA), positively charged polyacrylamide (PAM), neutral polyethylene glycol (PEG) and zwitterionic polymer poly(sulfobetaine) (pSB) are used to modify GO. The PCR specificity-enhancing ability increases in the following order: GO-PAA |
| Databáze: | OpenAIRE |
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