Cholinergic involvement in vascular and glucoregulatory actions of insulin in rats
| Autor: | André Nadeau, Martin Lévesque, Maryse Pitre, Hélène Bachelard, Marta Santuré |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Atropine
Male medicine.medical_specialty Time Factors Endocrinology Diabetes and Metabolism Glucose uptake medicine.medical_treatment Cholinergic Agents Vasodilation Rats Sprague-Dawley Internal medicine Diabetes mellitus Internal Medicine medicine Animals Insulin Muscle Skeletal Pancreatic hormone Chemistry medicine.disease Rats Blood pressure Endocrinology Glucose Glucose Clamp Technique Cholinergic medicine.drug |
| Zdroj: | Diabetes. 55(2) |
| ISSN: | 0012-1797 |
| Popis: | This study was designed to test the glucose metabolic and vasodilator actions of insulin in rats and its relation to cholinergic system–dependent mechanisms. The first group of rats had pulsed Doppler flow probes and intravascular catheters implanted to determine blood pressure, heart rate, and regional blood flows. Insulin sensitivity was assessed by the euglycemic-hyperinsulinemic clamp technique carried out in the absence or presence of atropine. The second group of rats was used to determine the cholinergic contribution to in vivo insulin-mediated glucose utilization in individual muscles. Glucose uptake was examined by using [ 3 H]2-deoxy-d-glucose. Muscarinic cholinergic blockade was found to significantly ( P = 0.002) reduce insulin sensitivity and to completely abrogate the renal ( P = 0.008) and hindquarter ( P = 0.02) vasodilator responses to euglycemic infusion of insulin. A significant reduction in insulin-stimulated in vivo glucose uptake was also noted in soleus ( P = 0.006), quadriceps ( P = 0.03), gastrocnemius ( P = 0.02), and extensor digitorum longus (EDL) ( P = 0.001) muscles, when insulin was infused at a rate of 4 mU · kg −1 · min −1 , whereas at the rate of 16 mU · kg −1 · min −1 , a significant reduction in glucose uptake was only observed in EDL ( P = 0.03) and quadriceps ( P = 0.01) muscles. Together, these results demonstrate a potential role for cholinergic involvement with physiological insulin actions in glucose clearance and blood flow regulation in rats. |
| Databáze: | OpenAIRE |
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