Comparison of Bacteroides thetaiotaomicron and Escherichia coli 16S rRNA gene expression signals
| Autor: | Mary L. Thorson, Matthew D. Mastropaolo, Ann M. Stevens |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Gene Expression Biology medicine.disease_cause Microbiology Genes Reporter RNA Ribosomal 16S Consensus Sequence Escherichia coli medicine Consensus sequence Bacteroides Internal transcribed spacer Promoter Regions Genetic Gene Genetics Base Sequence Genes rRNA Promoter Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Molecular biology Luminescent Measurements Photorhabdus Bacteroides thetaiotaomicron |
| Zdroj: | Microbiology. 155:2683-2693 |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | There are barriers to cross-expression of genes betweenBacteroidesspp. andEscherichia coli. In this study, alux-based reporter system was developed forBacteroidesand used to compare the promoter structure and function of aBacteroides thetaiotaomicron4001 (BT4001) 16S rRNA promoter with those ofE. coli in vivo. Analysis of the BT4001 sequences upstream of the 16S rRNA gene revealed the same overall structure known forE. coli16S rRNA promoters in that there were two promoters separated by ∼150 bp. However, the BT4001 16S rRNA promoter contains the proposedBacteroides−7 and −33 consensus sequences instead of theE. coli−10 and −35 consensus sequences. The biological activity of various configurations of the BT4001 16S rRNA promoter was analysed. Experiments pairing the BT4001 16S rRNA promoter with anE. coliRBS, and vice-versa, confirmed that gene expression between the two species is restricted at the level of transcription. InBacteroides, a difference in translation initiation also appears to limit expression of foreign genes. |
| Databáze: | OpenAIRE |
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