Surgical Skills Training with Cryopreserved Rat Stomachs

Autor: Jazmín A. García-Montes, Diana Pérez-Covarrubias, J. Alfredo Santibáñez-Salgado, Isabel Guadarrama-Sánchez, J. Luis Torre-Jaime, Shely Azrad-Daniel, Avelina Sotres-Vega, Jaime Villalba-Caloca
Rok vydání: 2016
Předmět:
Zdroj: Journal of Veterinary Medical Education. 43:420-426
ISSN: 1943-7218
0748-321X
DOI: 10.3138/jvme.0515-074r3
Popis: The objective of this study is to present a high-fidelity bench model of cryopreserved stomachs that can be used while learning surgical skills. Thirty stomachs were harvested from Wistar rats at the end of non-abdominal research studies. The stomachs were washed with cold saline solution and filled with hyaluronic acid solution. The organs were then placed into cryovials and cryopreserved at −30 °C for 60 days. The stomachs were thawed to room temperature on the day of the surgical skills practice and two full-thickness incisions were made. Reporting on their experiences, 22 participants (73.33%) felt that the cryopreserved stomach was identical to in vivo rat stomachs, 24 (80.00%) reported that the stomach was easy to handle, and 27 (90%) reported the tissue was non-friable. Moreover, 29 participants (96.6%) finished the suturing without tears and 100% recommended it as a biomaterial for surgical training. The cryopreserved stomach is a practical, reproducible, low-cost, and high-fidelity bench model that allows surgical fellows to learn how to handle a stomach and improve their surgical abilities before performing surgery on patients or laboratory animals.
Databáze: OpenAIRE