Inhibition of cell proliferation on lens capsules by 4197X-ricin A immunoconjugate
| Autor: | Peter J. Kelleher, Maureen P. Tarsio, Dominic Man-Kit Lam, Jared M. Emery, Joseph F. Tarsio |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Cell Culture Techniques Lens Capsule Crystalline Ricin Epithelium Immunotoxin In vivo medicine Humans Cytotoxicity Cell growth business.industry Immunotoxins fungi Antibodies Monoclonal Epithelial Cells Crystallins Sensory Systems In vitro Cell biology Immunoconjugate Ophthalmology medicine.anatomical_structure Cell culture Lens (anatomy) Surgery sense organs business Cell Division Follow-Up Studies |
| Zdroj: | Journal of cataract and refractive surgery. 23(2) |
| ISSN: | 0886-3350 |
| Popis: | Purpose: To evaluate the cytotoxicity of immunotoxin 4197X-ricin A (4197X-RA) and its ability to inhibit protein synthesis and human lens epithelial cell (LEC) proliferation on the inner surface of the lens capsule. Setting: Houston Biotechnology, Inc., The Woodlands, Texas. Methods: A cell culture system was established using human LECs as a model for the proliferation of remnant LECs that occurs during posterior capsule opacification (PCO) after extracapsular cataract extraction. The LEC culture system was also used in vitro for testing compounds that might inhibit this process in vivo. Human LECs were cultured on the surface of the original lens capsule fixed to collagen. Variability was reduced by dissecting each lens capsule into equivalent halves and exposing the segments to immunotoxin 4197X-RA. Results: Protein synthesis and LEC proliferation were almost completely inhibited at relatively low 4197X-RA concentrations after short exposure. The inhibitory effects persisted up to 3 weeks after withdrawal of the immunotoxin and after several media exchanges. Conclusion: Immunotoxin 4197X-RA may help prevent PCO after primary cataract surgery. |
| Databáze: | OpenAIRE |
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