Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study

Autor: Alana C. Conti, Rajtarun Madangopal, Kamran Avanaki, Bruce T. Hope, Kassem M. Makki, Veronica A Lennon, James I. Matchynski, Abbey L. Reppen, Shane A. Perrine, Rayyan Manwar, Alexander R. Woznicki, Karl Kratkiewicz
Rok vydání: 2021
Předmět:
CNR
contrast-to-noise ratio
PA
photoacoustic
QC1-999
Transgene
QC221-246
Photoacoustic imaging in biomedicine
lac operon
F/M-PAT
functional molecular photoacoustic tomography
Fear conditioning
DMSO
dimethyl sulfoxide
Stimulus (physiology)
X-gal
chemistry.chemical_compound
PBS
phosphate buffer saline
ML
medial-lateral
Cocaine
AP
anterior-posterior
Radiology
Nuclear Medicine and imaging
DV
dorsal-ventral
IL
infralimbic cortex
Prefrontal cortex
GRIN
gradient-index
Neuronal ensemble
PL
prelimbic cortex
OCT
optical coherence tomography
US
ultrasound
Chemistry
Fos
Physics
Acoustics. Sound
mPFC
medial prefrontal cortex
Brain
OPO
optical parametric oscillator
QC350-467
SNR
signal-to-noise ratio
Optics. Light
Atomic and Molecular Physics
and Optics
nervous system
fMRI
functional magnetic resonance imaging
ANSI
American national standards institute
FOV
field-of-view
Photoacoustic imaging
Neuroscience
Ex vivo
Research Article
X-gal
beta-D-galactosidase
Zdroj: Photoacoustics, Vol 24, Iss, Pp 100297-(2021)
Photoacoustics
ISSN: 2213-5979
Popis: Highlights • We developed a photoacoustic modality for direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats. • X-gal product provided exogenous contrast for imaging of neuronal ensembles. • We detected Fos-expressing activated neuronal ensembles after either cocaine administration or a shock-tone pairing paradigm.
Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large field-of-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an ex vivo imaging method in the Wistar Fos-LacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fos-expressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction.
Databáze: OpenAIRE
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