Emergence of nuclear heparanase induces differentiation of human mammary cancer cells
| Autor: | Esra Gunduz, Minoru Haisa, Yoko Tabuchi, Noriaki Tanaka, Keizou Ookawa, Yoshio Naomoto, Mehmet Gunduz, Motowo Nakajima, Dai Kitamoto, Munenori Takaoka, Junji Matsuoka, Tetsuji Nobuhisa, Hitoshi Nagatsuka |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Programmed cell death
Cellular differentiation Mesenchyme Genetic Vectors Biophysics Breast Neoplasms Biology Transfection Biochemistry Cell Line Tumor medicine Humans Heparanase Molecular Biology Epithelial cell differentiation Glucuronidase Cell Nucleus Cell Differentiation Cell Biology Lipids Cell biology medicine.anatomical_structure Cytoplasm Cancer cell Female Stem cell |
| Zdroj: | Biochemical and biophysical research communications. 331(1) |
| ISSN: | 0006-291X |
| Popis: | The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect