Culture of human breast cancer cell line (MDA-MB-231) on fibronectin-coated surface induces pro-matrix metalloproteinase-9 expression and activity
| Autor: | Paromita Roy Choudhury, Hrishikesh Sil, Amitava Chatterjee, Triparna Sen, Gargi Maity, Kirat Kumar Ganguly |
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| Rok vydání: | 2010 |
| Předmět: |
Integrin
Blotting Western Breast Neoplasms Electrophoretic Mobility Shift Assay Enzyme-Linked Immunosorbent Assay Matrix metalloproteinase Protein Serine-Threonine Kinases Focal adhesion Extracellular matrix Transactivation Cell Movement Cell Line Tumor Cell Adhesion Humans Integrin-linked kinase RNA Messenger Phosphorylation Cell Proliferation Enzyme Precursors biology Reverse Transcriptase Polymerase Chain Reaction NF-kappa B General Medicine Flow Cytometry Cell biology Fibronectins Fibronectin Matrix Metalloproteinase 9 Focal Adhesion Protein-Tyrosine Kinases biology.protein Female Signal transduction Phosphatidylinositol 3-Kinase Integrin alpha5beta1 Signal Transduction |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 32(1) |
| ISSN: | 1423-0380 |
| Popis: | Interaction between cell surface integrin receptors with extracellular matrix (ECM) plays an important role in cell survival, proliferation, and migration including tumor development and invasion. Binding of ECM to integrins initiates intracellular signaling cascades, modulating expression and activity of different matrix metalloproteinases (MMPs) which is important in ECM degradation. The present study investigates fibronectin–integrin-mediated signaling and thereby modulation of MMPs expression and activity in human breast cancer cell line, MDA-MB-231. Culture of MDA-MB-231 cells on fibronectin (FN) induced expression and activity of pro-matrixmetalloproteinase-9 (MMP-9). Appreciable reduction of FN-induced pro-MMP-9 activity was observed in anti-α5 antibody treated cells. Inhibitor studies revealed that inhibitors of phosphatidyl inositiol-3-kinase (PI-3K), and nuclear factor kappa B (NF-κB) inhibited FN-induced pro-MMP-9 activity. FN increased tyrosine phosphorylation of focal adhesion kinase (FAK), integrin linked kinase (ILK), and PI-3K in MDA-MB-231 cells. FN-induced the transactivation of MMP-9 promoter by enhancing DNA binding activity of NF-κB and Sp1. Wound healing assay showed faster migration of MDA-MB-231cells grown on fibronectin-coated as surface as compared to control. Our findings indicated that culture of MDA-MB-231 on fibronectin perhaps send signals via fibronectin–integrin-mediated signaling pathways recruiting FAK, PI-3K, ILK, NF-κB, and modulate expression and activation of pro-MMP-9. These observations may enrich fundamental aspects of cancer biology especially role of α5β1 integrin in regulation of MMPs expression and activity. |
| Databáze: | OpenAIRE |
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