Rapid glucocorticoid effects on the expression of hippocampal neurotransmission-related genes
| Autor: | Marian Joëls, E.R. de Kloet, P.J. Steenbergen, M.C. Morsink, N.G. van Gemert, Nicole A. Datson |
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| Přispěvatelé: | Structural and Functional Plasticity of the nervous system (SILS, FNWI) |
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty In situ hybridization Neurotransmission Hippocampal formation Biology Hippocampus chemistry.chemical_compound Mineralocorticoid receptor Glucocorticoid receptor Hormone Antagonists Corticosterone Internal medicine Gene expression medicine Animals Drug Interactions RNA Messenger Rats Wistar Casein Kinase II Molecular Biology Glucocorticoids Monoamine Oxidase In Situ Hybridization Analysis of Variance General Neuroscience Rats Mifepristone Endocrinology Receptors Mineralocorticoid chemistry Gene Expression Regulation Shaw Potassium Channels Neurology (clinical) Glucocorticoid Developmental Biology medicine.drug |
| Zdroj: | Brain Research, 1150, 14-20. Elsevier |
| ISSN: | 1872-6240 0006-8993 |
| Popis: | We previously assessed corticosterone mediated gene expression in acute explant hippocampal slices and found over 200 responsive genes 1, 3 and 5 h after glucocorticoid receptor (GR) activation by a brief corticosterone pulse. Interestingly, 1 h after GR activation all genes were downregulated, many of which are involved in hippocampal neurotransmission and plasticity. The aim of the current experiment was 1) to measure the expression of several of these neurotransmission-related genes that were corticosterone-responsive 1 h after GR-activation in an in vivo setting, 2) to elucidate in which hippocampal subregion these expression changes take place and 3) to assess the specificity of regulation by activated GRs. For this purpose, rats were subcutaneously injected with vehicle, corticosterone or corticosterone pretreated with GR-antagonist RU38486. One hour after the corticosterone injections, mRNA expression levels of 5 selected genes were measured using in situ hybridization. The mineralocorticoid receptor (MR), MAO-A, casein kinase 2 and voltage dependent potassium mRNA's, but not dynein mRNA, were rapidly downregulated in vivo after corticosterone administration in hippocampal subregions. Furthermore, RU38486 pretreatment reversed in all cases these effects, illustrating the GR-specificity of transcriptional regulation by corticosterone. The results are important for understanding the role of GR in pleiotropic control of hippocampal neurotransmission and plasticity, which is characterized by recovery of function transiently raised by excitatory input. |
| Databáze: | OpenAIRE |
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