Chimerism in PCR products from a multigene family
| Autor: | D. Bhavsar, H. Zheng, J.W. Drysdale |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Genetics
education.field_of_study Base Sequence Recombinant Fusion Proteins Population Pcr cloning Molecular Sequence Data Biophysics Cell Biology DNA Biology Biochemistry Polymerase Chain Reaction Conserved sequence law.invention Chimera (genetics) law Multigene Family Ferritins Humans Cloning Molecular education Molecular Biology Polymerase chain reaction |
| Zdroj: | Biochemical and biophysical research communications. 205(1) |
| ISSN: | 0006-291X |
| Popis: | This paper describes a potential hazard in obtaining sequences of a multigene family by amplifying the mixed population by the polymerase chain reaction with primers from conserved sequences. We describe a product which appears to be a chimera formed during the PCR reaction from two different but related sequences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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