Differential Expression of CircRNAs in Embryonic Heart Tissue Associated with Ventricular Septal Defect
| Autor: | Jing Yin, Heng Liu, Zhang Bin Yu, Mengmeng Li, Yin Hu, Shu Ping Han, Juan Wang, Xiao Hui Chen, Xing Yun Wang, Jing Xu, Bin Zhuang |
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| Rok vydání: | 2018 |
| Předmět: |
Heart Septal Defects
Ventricular 0301 basic medicine congenital hereditary and neonatal diseases and abnormalities Heart development Embryonic heart Microarray Significant difference RNA Circular General Medicine Biology Molecular biology Fold change Reverse transcription polymerase chain reaction MicroRNAs 03 medical and health sciences 030104 developmental biology Case-Control Studies microRNA Humans RNA Differential expression Oligonucleotide Array Sequence Analysis |
| Zdroj: | International Journal of Medical Sciences. 15:703-712 |
| ISSN: | 1449-1907 |
| DOI: | 10.7150/ijms.21660 |
| Popis: | Objectives: To explore and validate the differential expression of circRNAs in the myocardium of congenital ventricular septal defect (VSD) and to explore a new avenue of research regarding the pathological mechanisms of VSD. Methods: We detected circRNAs expression profiles in heart tissues taken from six aborted fetuses with VSD and normal group using circRNA microarray. Some differentially expressed circRNAs were studied by bioinformatics analysis. Finally, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results. Results: This study found abundant circRNAs in the myocardium taken from individuals in the normal group and the VSD group. After that, totally 6234 differentially expressed circRNAs between the normal group and the VSD group were confirmed (Fold change ≥ 2.0; p < 0.05). Then, this research carried out bioinformatics analysis and predicted the potential biological functions of circRNAs. Finally, the over-expression of hsa_circRNA_002086 and under-expression of hsa_circRNA_007878, hsa_circRNA_100709, hsa_circRNA_101965, hsa_circRNA_402565 were further validated by qRT-PCR. Conclusions: There is a significant difference in expression of the circRNA in cardiac tissue from VSD group compared to the normal group. Combined with the microarray results and previous researches, circRNAs may contribute to the occurrence of VSD by acting as miRNA sponges or by binding proteins, these possible roles for circRNAs in VSD require elucidation in additional studies. |
| Databáze: | OpenAIRE |
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