Genetic Manipulation of Mycobacterium tuberculosis
| Autor: | Steven Tandberg, Michelle H. Larsen, Karolin Biermann, Tsugunda Hsu, William R. Jacobs |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Tuberculosis Biology Microbiology Mycobacterium tuberculosis Transduction (genetics) chemistry.chemical_compound Plasmid Transduction Genetic Virology medicine Humans Genetics General Medicine biology.organism_classification medicine.disease genomic DNA Electroporation chemistry Genetic Techniques Mutagenesis Nucleic acid DNA Transposable Elements Parasitology Transposon mutagenesis DNA |
| Zdroj: | Current Protocols in Microbiology |
| ISSN: | 1934-8533 |
| Popis: | This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed. |
| Databáze: | OpenAIRE |
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