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Additional file 1: Figure S1. Adherent junction pathway in tumor-infiltrating PMN-MDSCs. The upregulated genes in PMN-MDSCs from 2 patients were uploaded in DAVID to identify the biological pathways. Adherent junction is the top KEGG pathway regulated in PMN-MDSCs, compared with APCs within the TME. The black ovals highlight the KEGG identified functional pathways that are regulated by PMN-MDSCs, within the TME. The functional consequences of related genes are shown in red. Figure S2. HIF-1 signaling pathway in tumor-infiltrating I-MDSCs. The downregulated genes in PMN-MDSCs, compared with I-MDSCs from two patients were uploaded in DAVID to identify the biological pathways. HIF-1 signaling is the top KEGG pathway regulated in I-MDSCs, within the TME. The black ovals highlight the KEGG identified functional pathways that are regulated by I-MDSCs, within the TME. The functional consequences of related genes are shown in red. Figure S3. Validation of differential gene expression and functional network analyses of APCs, PMN-MDSCs and I-MDSCs in CRC patients. Heat maps show the TPM representing fold change to the mean expression of WNT signaling, SNARE signaling and JNK pathway activation in I-MDSCs (A). Heat maps show the TPM representing fold change relative to the mean expression of colorectal cancer-, cell migration-, NFκB-, IL-1β production-related genes in PMN-MDSCs, compared with APCs (B). Heat map shows the TPM representing fold change relative to the mean expression of tumor progression-, migration and metastasis- and DNA methylation-related genes in PMN-MDSCs (C). Results obtained from four CRC patients (#09, #12, #13, and #16). Figure S4. CD11a expression in tumor-infiltrating PMN-MDSCs. Heat map shows the TPM representing fold change relative to the mean expression of CD11a gene (ITGAL) in PMN-MDSCs (A). Cells isolated from TT of #07 and #08 patients were stained for myeloid cell markers and CD11a, and analyzed by flow cytometry. Representative flow cytometric plots show the gating strategy employed to identify I-MDSCs and PMN-MDSCs expressing CD11a (B). |
