An investigation of genetic variation within a series of congenic strains of mice

Autor: P. Totman, D. P. Lovell, J. B. Greig, S. W. Bigelow, H. A. Hoffman, D. W. Nebert, Michael F. W. Festing
Rok vydání: 1984
Předmět:
Zdroj: Laboratory Animals. 18:291-297
ISSN: 1758-1117
0023-6772
DOI: 10.1258/002367784780958231
Popis: Summary 2 congenic strains of mice, B6N.AKN-Ahk and D2N.B6N-Ahb, imported from the USA, were found to be either segregating or fixed for an incorrect allele at a number of biochemical loci. B6N.AKNAh\ supposedly congenic with C57BL/6N, had the wrong genotype at 6 out of 12 biochemical loci; D2N.B6N_Ahb, supposedly congenic with DBA/2N, was segregating at 3 out of 9 loci. There was genetic variation in mandible shape within the 2 strains but no abnormal coat colours were found and no hybrid vigour in breeding performance was detected. Analyses in the USA confirmed these results and showed that 2 other congenic strains, C3N.D2N-Ahd and AKN.B6J-Ah\ were also segregating at a number of loci. Some of the alleles found in the C3N.D2N-Ahd mice must be the result of a genetic contamination. The simplest explanation for this breakdown in the backcrossing programme is genetic contamination with other congenic strains or recombinant inbred lines under development in the same laboratory. These findings emphasize the importance of continual genetic monitoring of all genetic stocks at regular intervals and in particular during the development of congenic and recombinant lines. This paper describes the detection of genetic variation in 2 congenic strains of mice sent to the MRC Laboratory Animals Centre (now known as the MRC Experimental Embryology and Teratology Unit) and the subsequent investigations carried out in the UK and USA. Congenic strains are developed by repeatedly backcrossing a gene on to an inbred background (Bailey, 1981). This particular set were developed to
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