High-yield production of active recombinant S. simulans lysostaphin expressed in E. coli in a laboratory bioreactor
| Autor: | Barış Binay, John M. Woodley, Aişe Ünlü, Zeynep Efsun Duman-Özdamar, Hayriye Unal |
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| Rok vydání: | 2021 |
| Předmět: |
Glycerol
0106 biological sciences Staphylococcus aureus Auto-induction medium Scale-up Staphylococcus Genetic Vectors Gene Expression E. coli expression system medicine.disease_cause 01 natural sciences law.invention 03 medical and health sciences Bioreactors Bacteriocin law 010608 biotechnology Escherichia coli medicine Bioreactor Food science Cloning Molecular 030304 developmental biology 0303 health sciences biology Chemistry Lysostaphin Laboratory bioreactor biology.organism_classification Bioproduction L- (+) arabinose Recombinant Proteins Anti-Bacterial Agents Culture Media Glucose Fermentation Recombinant DNA Bacteria Biotechnology |
| Zdroj: | Duman-Özdamar, Z E, Ünlü, A, Ünal, H, Woodley, J M & Bi̇nay, B 2021, ' High-yield production of active recombinant S. simulans lysostaphin expressed in E. coli in a laboratory bioreactor ', Protein Expression and Purification, vol. 177, 105753 . https://doi.org/10.1016/j.pep.2020.105753 |
| ISSN: | 1046-5928 |
| DOI: | 10.1016/j.pep.2020.105753 |
| Popis: | Staphylococcus aureus (S. aureus), which has developed multidrug resistance, leads to many healthcare-associated infections resulting in significant medical and economic losses. Therefore, the development of new efficient strategies to deal with these bacteria has been gaining importance. Lysostaphin is a peptidoglycan hydrolase that has considerable potential as a bacteriocin. However, there have been few reported optimization and scale-up studies of the lysostaphin bioproduction process. Our preliminary results have revealed that the composition of auto-induction media at 30 °C increases the produced lysostaphin around 10-fold in shake flasks. In this study, achieving higher yields for recombinant lysostaphin in E. coli at a laboratory scale has been the aim, through the use of auto-induction media. Optimized medium composition and fermentation parameters were transferred to a laboratory-scale bioreactor. The tested conditions improved protein yields up to 184 mg/L in a 3 L stirred bioreactor and the productivity was improved 2-fold in comparison to previously published reports. Furthermore, this study also showed that lysostaphin is an effective bacteriocin on both commercially available and isolated S. aureus strains. These results will contribute to future larger-scale production of lysostaphin via the proposed fermentation conditions. |
| Databáze: | OpenAIRE |
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