Effects of feeding Bt maize to sows during gestation and lactation on maternal and offspring immunity and fate of transgenic material
| Autor: | R. Paul Ross, Stefan G. Buzoianu, Eva Gelencsér, Gillian E. Gardiner, Peadar G. Lawlor, Orla O'Donovan, Andras Nagy, Erika Szabó, Gabriella Ujhelyi, Mary C. Rea, Maria Walsh |
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| Přispěvatelé: | European Union, Teagasc Walsh Fellowship Programme |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
System
Animal Nutrition Allergy Swine Lymphocyte Bacillus-thuringiensis Biochemistry Antibody Specificity Pregnancy Lactation Genetically-modified maize Mechanisms Offspring immunity Cry1Ab Immune Response Animal Management 2. Zero hunger 0303 health sciences Multidisciplinary Allergy and Hypersensitivity food and beverages Agriculture 04 agricultural and veterinary sciences Plants Genetically Modified Antibodies Bacterial 040401 food science medicine.anatomical_structure Medicine Cytokines Gestation Pigs Female Genetic Engineering Research Article Biotechnology medicine.medical_specialty Offspring Science Animal Types Immunology Cereals Genetically Modified Foods Crops Immune function Large Animals Biology Insemination Zea mays Peripheral blood mononuclear cell Non-GM Andrology 03 medical and health sciences 0404 agricultural biotechnology Internal medicine medicine Sows Animals Cytokine Feces 030304 developmental biology Inflammation Immunity DNA medicine.disease Maize Diet Endotoxins Endocrinology Health-risks Genetically modified maize Leukocytes Mononuclear Veterinary Science |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 10, p e47851 (2012) |
| Popis: | peer-reviewed Background: We aimed to determine the effect of feeding transgenic maize to sows during gestation and lactation on maternal and offspring immunity and to assess the fate of transgenic material. Methodology/Principal Findings: On the day of insemination, sows were assigned to one of two treatments (n = 12/treatment); 1) non-Bt control maize diet or 2) Bt-MON810 maize diet, which were fed for ~143 days throughout gestation and lactation. Immune function was assessed by leukocyte phenotyping, haematology and Cry1Ab-specific antibody presence in blood on days 0, 28 and 110 of gestation and at the end of lactation. Peripheral-blood mononuclear cell cytokine production was investigated on days 28 and 110 of gestation. Haematological analysis was performed on offspring at birth (n = 12/treatment). Presence of the cry1Ab transgene was assessed in sows' blood and faeces on day 110 of gestation and in blood and tissues of offspring at birth. Cry1Ab protein presence was assessed in sows' blood during gestation and lactation and in tissues of offspring at birth. Blood monocyte count and percentage were higher (P |
| Databáze: | OpenAIRE |
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