Genistein Enhances Antigen-Specific Cytokine Production in Female DO11.10 Transgenic Mice
| Autor: | Shigeru Yamamoto, Kaori Mitsuya, Tohru Sakai, Tatsushi Komatsu, Mari Kogiso |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Genetically modified mouse medicine.medical_specialty Ovalbumin Ratón Transgene medicine.medical_treatment Medicine (miscellaneous) Genistein Enzyme-Linked Immunosorbent Assay Mice Transgenic Biology Lymphocyte Activation Interferon-gamma Mice chemistry.chemical_compound Sex Factors Antigen Internal medicine medicine Animals Anticarcinogenic Agents Antigens Mice Inbred BALB C Nutrition and Dietetics food and beverages T lymphocyte Flow Cytometry Endocrinology Cytokine chemistry biology.protein Cytokines Female Interleukin-4 |
| Zdroj: | Journal of Nutritional Science and Vitaminology. 52:327-332 |
| ISSN: | 1881-7742 0301-4800 |
| DOI: | 10.3177/jnsv.52.327 |
| Popis: | Genistein is a phytoestrogen contained at high levels in soy products and has been shown to regulate immunoresponse. In this study, we evaluated the effects of genistein on the production of cytokines from antigen (Ag)-specific T cells using DO11.10 transgenic mice because the direct effect of genistein on Ag-specific cytokine production has not been elucidated. The oral administration of 20 mg/kg genistein increased IFN-gamma and IL-4 production from DO11.10+ T cells in response to ovalbumin (OVA)323-339 peptide in female DO11.10 mice. Analysis of intracellular cytokine synthesis revealed that the percentages of cytokine-producing cells in the control and genistein-treated groups were not different, indicating that increased cytokine production occurred at the single-cell level. In contrast to the female mice, genistein did not increase cytokine production in male mice, suggesting that the effect of genistein on cytokine production is gender-dependent. |
| Databáze: | OpenAIRE |
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