Assessing a multiplex-targeted proteomics approach for the clinical diagnosis of periodontitis using saliva samples
| Autor: | Valérie Orti, Brenda Mertens, Sylvain Lehmann, Philippe Gibert, Christophe Hirtz, Jérôme Vialaret, Aroa Relaño-Ginés, Dominique Deville de Périère |
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| Přispěvatelé: | Université de Montpellier (UM), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Plateforme de Protéomique Clinique, CHU Saint-Eloi |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Saliva [SDV]Life Sciences [q-bio] Clinical Biochemistry Gastroenterology Analytical Chemistry 03 medical and health sciences 0302 clinical medicine proteomics Internal medicine medicine Humans Aggressive periodontitis Multiplex General Pharmacology Toxicology and Pharmaceutics periodontitis mass spectrometry Periodontitis Proteomic Profile business.industry Hemopexin 030206 dentistry General Medicine clinical chemistry medicine.disease 3. Good health Medical Laboratory Technology 030104 developmental biology Biomarker (medicine) saliva biochemistry business oral pathology Apolipoprotein H |
| Zdroj: | Bioanalysis Bioanalysis, Future Science, 2018, 10 (1), pp.35-45. ⟨10.4155/bio-2017-0218⟩ |
| ISSN: | 1757-6180 1757-6199 |
| DOI: | 10.4155/bio-2017-0218⟩ |
| Popis: | International audience; Aim: The present study focused on the research of new biomarkers based on the liquid chromatography-multiple-reaction monitoring (MRM) proteomic profile in whole saliva of patients with periodontitis compared with periodontal healthy patients. Methods: A 30-min multiplexed liquid chromatography-MRM method was used for absolute quantification of 35 plasma biomarkers in saliva from control patients and patients with periodontitis. Results: Three proteins namely hemopexin, plasminogen and α-fibrinogen were shown to be clearly related to the presence of periodontitis compared with healthy patients. Apolipoprotein H was found to discriminate for the first time chronic and aggressive periodontitis. Conclusion: Our results indicate that this innovative MRM method could be used to screen for periodontitis in clinical environment. Furthermore, apolipoprotein H was found to be a discriminant biomarker of aggressive periodontitis. |
| Databáze: | OpenAIRE |
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