An Improved Method With High Specificity forKIR2DL1Functional Allele Typing
| Autor: | MaCal Tuggle, Wing Leung, Sarah Schell, Rafijul Bari |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Genetics
Genotype biology Biochemistry (medical) Clinical Biochemistry Single-nucleotide polymorphism Sequence Analysis DNA Polymorphism Single Nucleotide Sensitivity and Specificity law.invention Transmembrane domain Receptors KIR KIR2DL1 law Receptors KIR2DL1 biology.protein Humans Typing Antibody Allele Alleles Polymerase chain reaction |
| Zdroj: | Laboratory Medicine. 46:207-213 |
| ISSN: | 1943-7730 0007-5027 |
| DOI: | 10.1309/lmmn1k6sbrjcgd9j |
| Popis: | As new killer-cell immunoglobulin-like receptor (KIR) alleles are discovered, a challenge in KIR typing is maintaining sensitivity and specificity. A single nucleotide polymorphism assay can be used to type functional KIR2DL1 alleles. We improved recently on the earlier method by using a higher-specificity assay. The major modifications include the development of sequence-specific primers to selectively amplify the transmembrane domain of all known KIR2DL1 alleles via polymerase chain reaction with sequence-specific primers (PCR-SSP), and using the PCR products as the template for a revised KIR2DL1 functional allele-typing assay. This modified method allows high-throughput typing with high specificity. |
| Databáze: | OpenAIRE |
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