Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus
| Autor: | Sarah Talley, Niklas Bachmann, Adarsh Dharan, Edward M. Campbell, Virginia Zwikelmaier |
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| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
Microbiology (medical) Cytoplasm Indoles Phenylalanine Immunology Active Transport Cell Nucleus HIV Infections Virus Replication Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Capsid Genetics medicine Humans Nuclear pore 030304 developmental biology Cell Nucleus 0303 health sciences 030306 microbiology Chemistry Macrophages HEK 293 cells Reverse Transcription Cell Biology Reverse transcriptase Cell biology Cell nucleus HEK293 Cells medicine.anatomical_structure Viral replication Host-Pathogen Interactions HIV-1 Nuclear Pore Capsid Proteins Nuclear transport HeLa Cells |
| Zdroj: | Nature Microbiology. 5:1088-1095 |
| ISSN: | 2058-5276 |
| Popis: | Retroviral infection involves the reverse transcription of the viral RNA genome into DNA, which is subsequently integrated into the host cell genome. Human immunodeficiency virus type 1 (HIV-1) and other lentiviruses mediate the infection of non-dividing cells through the ability of the capsid protein1 to engage the cellular nuclear import pathways of the target cell and mediate their nuclear translocation through components of the nuclear pore complex2–4. Although recent studies have observed the presence of the capsid protein in the nucleus during infection5–8, reverse transcription and disassembly of the viral core have conventionally been considered to be cytoplasmic events. Here, we use an inducible nuclear pore complex blockade to monitor the kinetics of HIV-1 nuclear import and define the biochemical staging of these steps of infection. Surprisingly, we observe that nuclear import occurs with relatively rapid kinetics ( |
| Databáze: | OpenAIRE |
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