Sensitivity to expression levels underlies differential dominance of a putative null allele of the Drosophila tβh gene in behavioral phenotypes
| Autor: | Björn Brembs, Christine Damrau, Julien Colomb |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Heredity Genetic Linkage Mutant Social Sciences Homozygosity 590 Tiere (Zoologie) Mixed Function Oxygenases Animals Genetically Modified 0302 clinical medicine Gene expression Medicine and Health Sciences Drosophila Proteins Psychology Biology (General) Dominance (genetics) Genetics Heterozygosity biology General Neuroscience Null allele Phenotype Phenotypes Drosophila melanogaster X-Linked Traits Sex Linkage Female General Agricultural and Biological Sciences Research Article Genotype QH301-705.5 Tyramine General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Receptors Biogenic Amine Animals Allele Gene Octopamine Alleles Clinical Genetics Behavior General Immunology and Microbiology Biology and Life Sciences 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::570 Biowissenschaften Biologie biology.organism_classification 030104 developmental biology Genetic Loci Mutation ddc:590 030217 neurology & neurosurgery |
| Zdroj: | PLoS Biology, Vol 19, Iss 5, p e3001228 (2021) PLoS Biology |
| DOI: | 10.17169/refubium-31522 |
| Popis: | The biogenic amine octopamine (OA) and its precursor tyramine (TA) are involved in controlling a plethora of different physiological and behavioral processes. The tyramine-β-hydroxylase (tβh) gene encodes the enzyme catalyzing the last synthesis step from TA to OA. Here, we report differential dominance (from recessive to overdominant) of the putative null tβhnM18 allele in 2 behavioral measures in Buridan’s paradigm (walking speed and stripe deviation) and in proboscis extension (sugar sensitivity) in the fruit fly Drosophila melanogaster. The behavioral analysis of transgenic tβh expression experiments in mutant and wild-type flies as well as of OA and TA receptor mutants revealed a complex interaction of both aminergic systems. Our analysis suggests that the different neuronal networks responsible for the 3 phenotypes show differential sensitivity to tβh gene expression levels. The evidence suggests that this sensitivity is brought about by a TA/OA opponent system modulating the involved neuronal circuits. This conclusion has important implications for standard transgenic techniques commonly used in functional genetics. Differential dominance occurs when genes associated with several phenotypes (pleiotropic genes) show different modes of inheritance (e.g., recessive, dominant or overdominant) depending on the phenotype. This study reveals that differential sensitivity to gene expression levels can mediate differential dominance, which can be a significant challenge for standard transgenic techniques commonly used to elucidate gene function. |
| Databáze: | OpenAIRE |
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