Overexpression of periostin and distinct mesothelin forms predict malignant progression in a rat cholangiocarcinoma model
| Autor: | Miguel Á. Manzanares, Deanna J.W. Campbell, Alphonse E. Sirica, Gabrielle T. Maldonado |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Liver tumor Hepatology biology endocrine system diseases business.industry Original Articles Periostin medicine.disease 3. Good health 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Stroma Tumor progression 030220 oncology & carcinogenesis Cancer cell Cancer research medicine biology.protein Mesothelin Original Article business Immunostaining Intrahepatic Cholangiocarcinoma |
| Zdroj: | Hepatology Communications |
| ISSN: | 2471-254X |
| Popis: | Periostin and mesothelin have each been suggested to be predictors of poor survival for patients with intrahepatic cholangiocarcinoma, although the clinical prognostic value of both of these biomarkers remains uncertain. The aim of the current study was to investigate these biomarkers for their potential to act as tumor progression factors when assessed in orthotopic tumor and three-dimensional culture models of rat cholangiocarcinoma progression. Using our orthotopic model, we demonstrated a strong positive correlation between tumor and serum periostin and mesothelin and increasing liver tumor mass and associated peritoneal metastases that also reflected differences in cholangiocarcinoma cell aggressiveness and malignant grade. Periostin immunostaining was most prominent in the desmoplastic stroma of larger sized more aggressive liver tumors and peritoneal metastases. In comparison, mesothelin was more highly expressed in the cholangiocarcinoma cells; the slower growing more highly differentiated liver tumors exhibited a luminal cancer cell surface immunostaining for this biomarker, and the rapidly growing less differentiated liver and metastatic tumor masses largely showed cytoplasmic mesothelin immunoreactivity. Two molecular weight forms of mesothelin were identified, one at ∼40 kDa and the other, a more heavily glycosylated form, at ∼50 kDa. Increased expression of the 40-kDa mesothelin over that of the 50 kDa form predicted increased malignant progression in both the orthotopic liver tumors and in cholangiocarcinoma cells of different malignant potential in three-dimensional culture. Moreover, coculturing of cancer-associated myofibroblasts with cholangiocarcinoma cells promoted overexpression of the 40-kDa mesothelin, which correlated with enhanced malignant progression in vitro. Conclusion: Periostin and mesothelin are useful predictors of tumor progression in our rat desmoplastic cholangiocarcinoma models. This supports their relevance to human intrahepatic cholangiocarcinoma. (Hepatology Communications 2018;2:155-172). |
| Databáze: | OpenAIRE |
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