The bat gene of Halobacterium halobium encodes a trans-acting oxygen inducibility factor
| Autor: | Felix Gropp, Mary C. Betlach |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Halobacterium salinarum
Multidisciplinary Sequence Homology Amino Acid biology Operon Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Phenotype Oxygen chemistry.chemical_compound chemistry Genes Bacterial Bacteriorhodopsins Genes Regulator Gene cluster RNA Messenger Trans-acting Gene DNA Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 91:5475-5479 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Oxygen and light affect the expression of the bacterioopsin gene (bop), which encodes a light-driven proton pump in the purple membrane of Halobacterium halobium. This response is thought to be mediated by a set of genes located adjacent to the bop gene. DNA fragments containing either the bop gene or the entire bop gene cluster reversed the phenotype of purple membrane-deficient strains with mutations in the bop gene. Purple membrane synthesis was constitutive in one of these strains transformed with the bop gene alone. The same strain transformed with the bop gene cluster was inducible by low oxygen tension. Moreover, another strain that constitutively expresses purple membrane remained constitutive when transformed with the bop gene alone but the phenotype of the strain changed to inducible when transformed with the bop gene cluster. Additional experiments have confirmed that one of the genes of the bop gene cluster, the bat gene, encodes a trans-acting factor that is necessary and sufficient to confer inducibility of purple membrane synthesis by low oxygen tension. |
| Databáze: | OpenAIRE |
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