Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture
| Autor: | Valéry V. Wittwer, Christoph Tappeiner, Markus Tschopp, Theo G. Seiler, Beatrice E. Frueh, Stefan Zimmerli |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents medicine.drug_class 030106 microbiology Antibiotics 610 Medicine & health Organ culture Microbiology Cornea Corneal Transplantation 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine Amphotericin B medicine Animals Humans Voriconazole biology Organ Preservation Antimicrobial biology.organism_classification Tissue Donors Anti-Bacterial Agents Culture Media Penicillin Ophthalmology Streptomycin 030221 ophthalmology & optometry Cattle Bacteria medicine.drug |
| Zdroj: | Seiler, Günter Theodor Michael; Tschopp, Markus; Zimmerli, Stefan; Tappeiner, Christoph; Wittwer, Valéry; Früh Epstein, Beatrice (2015). Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture. Cornea, 35(1), pp. 127-131. Lippincott Williams & Wilkins 10.1097/ICO.0000000000000671 |
| ISSN: | 0277-3740 |
| Popis: | PURPOSE Contamination with bacteria and/or fungi is a serious complication in organ-cultured corneas. Hence, antibiotic and antifungal agents are added to the culture medium. The concentration of different antimicrobial and antifungal additives to the media over time has so far not been investigated in detail and is the aim of this study. METHODS Nine human fresh corneoscleral discs were stored in corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium. In addition, the culture medium contained 1200 μg/mL penicillin G, 25 μg/mL amphotericin B, 120 μg/mL streptomycin, and 100 μg/mL voriconazole. The concentration of amphotericin B used was 10 times higher than in clinical routine to facilitate its detection. The cultures were kept at 37°C for 28 days. At days 0, 7, 14, 21, and 28, samples of the culture medium were harvested for analysis of antimicrobial concentrations by liquid chromatography and electrospray ionization tandem mass spectrometry. RESULTS During corneal storage, the concentration of all antibiotics and antifungal agents declined significantly. By day 28, penicillin G was reduced to 14% of the original concentration. Amphotericin B and streptomycin retained approximately 60% of the original concentration to the end of the experiment and voriconazole maintained stable concentrations after an initial decline to approximately 80% at 7 days. CONCLUSIONS Throughout the entire storage period, the concentrations of penicillin G, streptomycin, and voriconazole exceeded the minimum inhibitory concentrations of all common contaminants, obviating the need for a change of the medium for antimicrobial reasons. Based on the minimum inhibitory concentrations and our findings, the initial concentration of amphotericin B should be raised to 5 μg/mL. |
| Databáze: | OpenAIRE |
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