The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc
| Autor: | Domenico Flagiello, J. L. Becker, Rénald Delanoue, Kevin Legent, Joël Silber, P. Vaudin, N Godefroy, Annie Dutriaux |
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| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
animal structures Cell Survival Biology Aminopterin E2F2 Transcription Factor DACAPO Morphogenesis medicine Animals Drosophila Proteins Humans Wings Animal Molecular Biology Transcription factor Genetics Regulation of gene expression Cell Death Cell growth Gene Expression Regulation Developmental Nuclear Proteins Cell Biology Cell biology Imaginal disc Drosophila Ectopic expression Cell Division HeLa Cells Signal Transduction Transcription Factors medicine.drug |
| Zdroj: | Cell Death & Differentiation. 11:110-122 |
| ISSN: | 1476-5403 1350-9047 |
| Popis: | Links between genes involved in development, proliferation and apoptosis have been difficult to establish. In the Drosophila wing disc, the vestigial (vg) and the scalloped (sd) gene products dimerize to form a functional transcription factor. Ectopic expression of vg in other imaginal discs induces outgrowth and wing tissue specification. We investigated the role of the VG-SD dimer in proliferation and showed that vg antagonizes the effect of dacapo, the cyclin-cdk inhibitor. Moreover, ectopic vg drives cell cycle progression and in HeLa cultured cells, the VG-SD dimer induces cell proliferation per se. In Drosophila, ectopic vg induces expression of dE2F1 and its targets dRNR2 and string. In addition vg, but not dE2F1, interacts with and induces expression of dihydrofolate reductase (DHFR). Moreover, a decrease in VG or addition of aminopterin, a specific DHFR inhibitor, shift the dorso-ventral boundary cells of the disc to a cell death sensitive state that is correlated with reaper induction and DIAP1 downregulation. This indicates that vg in interaction with dE2F1 and DHFR is a critical player for both cell proliferation and cell survival in the presumptive wing margin area. |
| Databáze: | OpenAIRE |
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