Gestational age-dependent up-regulation of prostaglandin D synthase (PGDS) and production of PGDS-derived antiinflammatory prostaglandins in human placenta
| Autor: | Roberto Romero, Timothy A. Sato, Linda Adams, Maxwell C. Chang, Simon J. O'Carroll, Jeffrey Keelan, Tinnakorn Chaiworapongsa, Rachel J. A. Helliwell, Murray D. Mitchell, Ashmit Anand, K.W. Marvin |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Amniotic fluid Endocrinology Diabetes and Metabolism Placenta Clinical Biochemistry Blotting Western Receptors Prostaglandin Prostaglandin Context (language use) Gestational Age Biochemistry Prostaglandin-D synthase Chorioallantoic Membrane chemistry.chemical_compound Endocrinology Fetal membrane Pregnancy Internal medicine medicine Humans Protein Isoforms RNA Messenger Receptors Immunologic Receptor biology Prostaglandin D2 Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Amniotic Fluid Immunohistochemistry Lipocalins Intramolecular Oxidoreductases medicine.anatomical_structure chemistry biology.protein Prostaglandins Cytokines Female Signal transduction |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 91(2) |
| ISSN: | 0021-972X |
| Popis: | The importance of prostaglandin (PG) signaling pathways to the maintenance of pregnancy and initiation of labor is well recognized. However, the complexity of these pathways and the mechanism(s) of their coordinated regulation in physiological and pathological conditions are only now being appreciated.In this report we provide new evidence of a complete pathway for the biosynthesis and actions of PGD(2) and its metabolites within human gestational tissues.Using immunohistochemistry and Northern and Western blotting, we demonstrate the dynamic regulation of H-type PGD synthase (PGDS) in placenta during gestation; in contrast, L-type PGDS and its PG products were detected in amniotic fluid, with increased amounts associated with labor.Placental tissues were shown to express both forms of the PGD(2) receptor identified to date, D prostanoid(1) (DP(1)) and DP(2)/chemotactic receptor on type 2 helper T cells, with a distribution consistent with the villous placenta being a major target, as well as source, of PGD(2). In vitro, placental PGD(2) production was shown to be stimulated upon inflammatory activation, whereas PGD(2) and its J series metabolites exerted potent inhibitory effects on placental cytokine production.These findings suggest that PGDS-derived prostanoids play important physiological roles in the placenta, such as immunoregulation and feto-placental communication, while potentially having a regulatory role in the processes of parturition. |
| Databáze: | OpenAIRE |
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