17β-Estradiol attenuates saturated fatty acid diet-induced liver injury in ovariectomized mice by up-regulating hepatic senescence marker protein-30
| Autor: | Hiroshi Obayashi, Goji Hasegawa, Naoto Nakamura, Masahiro Yamazaki, Yoshito Itoh, Mitsuhiro Ohta, Akihito Ishigami, Mai Asano, Jo Kitawaki, Michiaki Fukui, Takeshi Okanoue, Yayoi Kagami, Koichi Iwasa, Naoki Maruyama, Takafumi Senmaru |
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| Rok vydání: | 2011 |
| Předmět: |
X-Box Binding Protein 1
Senescence medicine.medical_specialty Normal diet Biophysics Gene Expression Apoptosis Regulatory Factor X Transcription Factors CHOP Biology Biochemistry Mice Non-alcoholic Fatty Liver Disease Internal medicine medicine Animals Endoplasmic Reticulum Chaperone BiP Molecular Biology Liver injury Estradiol Caspase 3 Tumor Necrosis Factor-alpha Calcium-Binding Proteins Fatty Acids Intracellular Signaling Peptides and Proteins Cell Biology medicine.disease Diet Up-Regulation DNA-Binding Proteins Fatty Liver Mice Inbred C57BL Endocrinology Liver Saturated fatty acid Ovariectomized rat Female Tumor necrosis factor alpha hormones hormone substitutes and hormone antagonists Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 415:252-257 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2011.10.025 |
| Popis: | Senescence marker protein-30 (SMP30) plays an important role in intracellular Ca2+ homeostasis. The aim of the present study was to investigate the effects of estrogens on liver apoptotic damage and changes in SMP30 expression induced by a high saturated fatty acid diet (HSFD). Ovariectomized mice (OVX) and sham-operated mice (SHAM) were randomly divided into five groups: SHAM fed a normal diet (SHAM/ND), SHAM fed HSFD (SHAM/HSFD), OVX fed ND (OVX/ND), OVX fed HSFD (OVX/HSFD) and OVX fed HSFD with 17β-estradiol (E2) supplementation using an implanted slow-release pellet (OVX/HSFD + E2). After 8 weeks, markers of endoplasmic reticulum (ER) stress and apoptosis, and levels of tumor necrosis factor-α (TNFα and SMP30 expression were investigated. Compared with SHAM/ND, OVX/HSFD mice showed significantly increased spliced X-box protein-1 (s-XBP1), phosphorylated eukaryotic initiation factor-2α (p-eIF2α), glucose-regulated protein 78 (GPR78), C/EBP homologous protein (CHOP), cytosolic cytochrome c, caspase-3 activity, and TNFα, and significantly decreased SMP30. These differences in OVX/HSFD mice were restored to the levels of SHAM/ND mice by E2 supplementation. These results suggest that E2 supplementation attenuates HSFD-induced liver apoptotic death in ovariectomized mice by up-regulating SMP30. |
| Databáze: | OpenAIRE |
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