Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules
| Autor: | A Hannah Rachel Vasanthi, Narasimman Gurusamy, K. S. Jayachandran |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death Dioscorea bulbifera Saponin Pharmaceutical Science Cardioprotection 03 medical and health sciences chemistry.chemical_compound Western blot reoxygenation Lactate dehydrogenase Drug Discovery medicine H9c2 Protein kinase B chemistry.chemical_classification medicine.diagnostic_test biology hypoxia Diosgenin biology.organism_classification 030104 developmental biology chemistry Biochemistry diosgenin Original Article |
| Zdroj: | Pharmacognosy Magazine |
| ISSN: | 0973-1296 |
| Popis: | Background: Diosgenin, a steroidal saponin from plants, exhibits many biological potentials. Herein, the cardioprotective role of diosgenin is studied. Materials and Methods: The effect of diosgenin, isolated from Dioscorea bulbifera, was studied on hypoxia-reoxygenation (HR) in H9c2 cardiomyoblast cells. The amount of diosgenin in the plant extract was analyzed by high-performance thin layer chromatography using a solvent system comprising of chloroform:methanol:acetic acid:formic acid (13:4.5:1.5:1). Cardioprotection was checked by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Further, the release of lactate dehydrogenase, an enzyme released during cell death was checked. The proteins responsible for cell death (Bax) and cell survival (Bcl-2, hemeoxygenase-1 and Akt) were analyzed using Western blot to check the cardioprotective role of diosgenin. Conclusion: Supplementation of diosgenin mitigates HR injury, thereby exhibiting cardioprotective potential. SUMMARY The cardioprotective effect of Diosgenin was evidenced from the improved cell survival after hypoxia-reoxygenation injury demonstrated through MTT cell survival assay.The release of lactate dehydrogenase, an enzyme released during cell death was decreased by Diosgenin.Diosgenin upregulated the pro-survival molecules like B-cell lymphoma 2 (Bcl-2), heme oxygenase-1 and the phosphorylation of ATK (at serine 473); and at the same time pro-.death molecules like Bax was downregulated.Thus, Diosgenin as a plant based steroidal saponin is confirmed to mitigate ischemic reperfusion injury. Figure |
| Databáze: | OpenAIRE |
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