Evaluation of equine coronavirus fecal shedding among hospitalized horses

Autor: Fairfield T. Bain, Macarena G. Sanz, Nicola Pusterla, Jenifer R. Gold, J. F. Evermann, So Young Kwon
Rok vydání: 2019
Předmět:
Rotavirus
Gastrointestinal Diseases
lethargy
Standard Article
030204 cardiovascular system & hematology
Viral infection
Polymerase Chain Reaction
0403 veterinary science
Parvovirus
Lethargy
Feces
0302 clinical medicine
Enteric disease
Betacoronavirus 1
fever
Microscopy
04 agricultural and veterinary sciences
Standard Articles
Hospitalization
Infectious Diseases
PCR
Gastrointestinal disease
anorexia
gastrointestinal disease
Washington
medicine.medical_specialty
040301 veterinary sciences
Equine coronavirus
Infectious Disease
anesthesia
Electron
Teaching hospital
03 medical and health sciences
Internal medicine
medicine
Animals
Clinical significance
Veterinary Sciences
Horses
electron microscopy
General Veterinary
business.industry
medicine.disease
Microscopy
Electron
Good Health and Well Being
Horse Diseases
EQUID
Digestive Diseases
business
Zdroj: Journal of Veterinary Internal Medicine
Journal of veterinary internal medicine, vol 33, iss 2
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.15449
Popis: Background Currently, diagnosis of equine coronavirus (ECoV) relies on the exclusion of other infectious causes of enteric disease along with molecular detection of ECoV in feces or tissue. Although this approach is complete, it is costly and may not always be achievable. Objective We hypothesized that the overall fecal shedding of ECoV in hospitalized horses is low. Our objective was to determine whether systemically healthy horses and horses with gastrointestinal disorders shed ECoV in their feces at the time of admission to a referral hospital and after 48 hours of stress associated with hospitalization. Animals One-hundred thirty adult horses admitted to the Washington State University Veterinary Teaching Hospital for gastrointestinal disease (n = 65) or for imaging under anesthesia (n = 65) that were hospitalized for 48 hours. Owner consent was obtained before sampling. Methods Fecal samples were collected at admission and 48 hours later. Polymerase chain reaction (PCR) for ECoV and electron microscopy (EM) were performed on all samples. Results Only 1 of 258 fecal samples was PCR-positive for ECoV. Electron microscopy identified ECoV-like particles in 9 of 258 samples, parvovirus-like particles in 4 of 258 samples, and rotavirus-like particles in 1 of 258 samples. Conclusions and clinical importance The presence of ECoV in feces of hospitalized adult horses was low. Thus, fecal samples that are PCR-positive for ECoV in adult horses that have clinical signs consistent with this viral infection are likely to be of diagnostic relevance. The clinical relevance of the viruses observed using EM remains to be investigated.
Databáze: OpenAIRE
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