Lipid storage by adipose tissue macrophages regulates systemic glucose tolerance
Autor: | Sarah M. Nicoloro, Joseph C. Yawe, Myriam Aouadi, Pranitha Vangala, Michaela Tencerova, Yuefei Shen, Michael P. Czech, Jessica Cohen |
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Rok vydání: | 2014 |
Předmět: |
Blood Glucose
Male medicine.medical_specialty Very low-density lipoprotein Physiology Endocrinology Diabetes and Metabolism CD36 Adipose tissue macrophages Adipose tissue Mice Obese Mice Insulin resistance Physiology (medical) Internal medicine Glucose Intolerance medicine Animals Obesity RNA Small Interfering Cells Cultured chemistry.chemical_classification Lipoprotein lipase biology Macrophages Fatty acid Lipid metabolism Articles medicine.disease Lipid Metabolism Mice Inbred C57BL Lipoprotein Lipase Endocrinology chemistry Adipose Tissue biology.protein lipids (amino acids peptides and proteins) |
Zdroj: | American journal of physiology. Endocrinology and metabolism. 307(4) |
ISSN: | 1522-1555 |
Popis: | Proinflammatory pathways in adipose tissue macrophages (ATMs) can impair glucose tolerance in obesity, but ATMs may also be beneficial as repositories for excess lipid that adipocytes are unable to store. To test this hypothesis, we selectively targeted visceral ATMs in obese mice with siRNA against lipoprotein lipase (LPL), leaving macrophages within other organs unaffected. Selective silencing of ATM LPL decreased foam cell formation in visceral adipose tissue of obese mice, consistent with a reduced supply of fatty acids from VLDL hydrolysis. Unexpectedly, silencing LPL also decreased the expression of genes involved in fatty acid uptake (CD36) and esterification in ATMs. This deficit in fatty acid uptake capacity was associated with increased circulating serum free fatty acids. Importantly, ATM LPL silencing also caused a marked increase in circulating fatty acid-binding protein-4, an adipocyte-derived lipid chaperone previously reported to induce liver insulin resistance and glucose intolerance. Consistent with this concept, obese mice with LPL-depleted ATMs exhibited higher hepatic glucose production from pyruvate and glucose intolerance. Silencing CD36 in ATMs also promoted glucose intolerance. Taken together, the data indicate that LPL secreted by ATMs enhances their ability to sequester excess lipid in obese mice, promoting systemic glucose tolerance. |
Databáze: | OpenAIRE |
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