Specific Immunoassays for Placental Alkaline Phosphatase As a Tumor Marker
| Autor: | Raul C. Ribeiro, Mara Albonei Dudeque Pianovski, João Carlos Minozzo, Dodie L. Arnold, Giovanna Assis Rodrigues, Bonald C. Figueiredo, Patrícia Zancanella, Juliana F. Moura, Enzo Lalli, Sérvio Túlio Stinghen, Luis G. Callefe |
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| Přispěvatelé: | Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC), Instituto de Pesquisa Pelé Pequeno Principe, Division of Pediatric Hematology and Oncology, Department of Pediatrics, Universidade Federal do Paraná (UFPR), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Meharry Medical College, CPPI, Center for Research and Production of Immunoglobulins, Department of Hematology and Oncology and International Outreach Program, St Jude Children's Research Hospital, IPPP, Research Institute Pelé Pequeno Príncipe, Fundação Araucária-PR, CAPES-COFECUB. American Lebanese Syrian Associated Charities (ALSAC), NIH/NCMHD |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Article Subject
Health Toxicology and Mutagenesis lcsh:Biotechnology Phosphatase lcsh:Medicine [SDV.CAN]Life Sciences [q-bio]/Cancer Embryonal carcinoma 03 medical and health sciences 0302 clinical medicine Affinity chromatography lcsh:TP248.13-248.65 Genetics Medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology Tumor marker 0303 health sciences biology business.industry lcsh:R General Medicine medicine.disease Molecular biology Enzyme assay Placental alkaline phosphatase Polyclonal antibodies 030220 oncology & carcinogenesis Immunology embryonic structures biology.protein cardiovascular system Molecular Medicine Antibody business [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Biotechnology Research Article |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Hindawi Publishing Corporation, 2006, 2006 (5), pp.56087. ⟨10.1155/JBB/2006/56087⟩ Journal of Biomedicine and Biotechnology, Vol 2006 (2006) |
| ISSN: | 1110-7243 1110-7251 |
| DOI: | 10.1155/JBB/2006/56087 |
| Popis: | Human placental (hPLAP) and germ cell (PLAP-like) alkaline phosphatases are polymorphic and heat-stable enzymes. This study was designed to develop specific immunoassays for quantifying hPLAP and PLAP-like enzyme activity (EA) in sera of cancer patients, pregnant women, or smokers. Polyclonal sheep anti-hPLAP antibodies were purified by affinity chromatography with whole hPLAP protein (ICA-PLAP assay) or a synthetic peptide (aa 57–71) of hPLAP (ICA-PEP assay); the working range was0.1–11U/L and cutoff value was0.2U/L EA for nonsmokers. The intra- and interassay coefficients of variation were3.7%–6.5% (ICA-PLAP assay) and9.0%–9.9% (ICA-PEP assay). An insignificant cross-reactivity was noted for high levels of unheated intestinal alkaline phosphatase in ICA-PEP assay. A positive correlation between the regression of tumor size and EA was noted in a child with embryonal carcinoma. It can be concluded that ICA-PEP assay is more specific than ICA-PLAP, which is still useful to detect other PLAP/PLAP-like phenotypes. |
| Databáze: | OpenAIRE |
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