Characterization of virus DNA synthesized in KB cells infected with two temperature-sensitive mutants of adenovirus type 5
| Autor: | John S. Sussenbach, M. G. Kuijk, M. W. van Maarschalkerweerd |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
DNA Replication
DNA polymerase DNA polymerase II Mutant DNA Single-Stranded Virus Cell Line chemistry.chemical_compound Viral Proteins Virology Humans DNA clamp biology Circular bacterial chromosome Adenoviruses Human Carcinoma DNA replication DNA Helicases Temperature Molecular biology chemistry DNA Viral Mutation biology.protein Mouth Neoplasms DNA |
| Zdroj: | The Journal of general virology. 44(1) |
| ISSN: | 0022-1317 |
| Popis: | Summary KB cells were infected with H5ts36 or H5ts125, two adenovirus type 5 (Ad5) mutants with a temperature-sensitive synthesis of virus DNA. Infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. Shortly after the shift-down H5ts125-infected cells showed an accumulation of purely single-stranded DNA of virus origin, which was not observed in H5ts36-infected cells. This single-stranded DNA has been characterized by hybridization and sedimentation analysis. It was found that the single-stranded DNA was derived from both complementary strands and consisted of short fragments. The observation that the single-stranded DNA accumulates in H5ts125-infected cells under conditions in which the amount of DNA binding protein is reduced, suggests that the DNA-binding protein is not only involved in initiation, but also in elongation of nascent strands. |
| Databáze: | OpenAIRE |
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