Metabolic heterogeneity of human hepatocellular carcinoma: implications for personalized pharmacological treatment
Autor: | Martin Stockmann, Niklas Heucke, Johannes Eckstein, Nikolaus Berndt, Hermann-Georg Holzhütter, Tilo Wuensch, Robert Gajowski, David Meierhofer |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Nitrogen Systems biology Glucose uptake Biology liver Biochemistry 03 medical and health sciences chemistry.chemical_compound Genetic Heterogeneity 0302 clinical medicine Cell Line Tumor medicine Humans Molecular Biology Glycogen Liver Neoplasms Cell Biology Metabolism HCCS Models Theoretical medicine.disease Lipid Metabolism kinetic modeling Warburg effect Gene Expression Regulation Neoplastic 030104 developmental biology Glucose chemistry 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer cell Cancer research Carbohydrate Metabolism tumor metabolism metabolism mathematical model 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit Signal Transduction |
Zdroj: | The FEBS Journal |
DOI: | 10.17169/refubium-34143 |
Popis: | Metabolic reprogramming is a characteristic feature of cancer cells, but there is no unique metabolic program for all tumors. Genetic and gene expression studies have revealed heterogeneous inter- and intratumor patterns of metabolic enzymes and membrane transporters. The functional implications of this heterogeneity remain often elusive. Here, we applied a systems biology approach to gain a comprehensive and quantitative picture of metabolic changes in individual hepatocellular carcinoma (HCC). We used protein intensity profiles determined by mass spectrometry in samples of 10 human HCCs and the adjacent noncancerous tissue to calibrate Hepatokin1, a complex mathematical model of liver metabolism. We computed the 24-h profile of 18 metabolic functions related to carbohydrate, lipid, and nitrogen metabolism. There was a general tendency among the tumors toward downregulated glucose uptake and glucose release albeit with large intertumor variability. This finding calls into question that the Warburg effect dictates the metabolic phenotype of HCC. All tumors comprised elevated ��-oxidation rates. Urea synthesis was found to be consistently downregulated but without compromising the tumor's capacity for ammonia detoxification owing to increased glutamine synthesis. The largest intertumor heterogeneity was found for the uptake and release of lactate and the size of the cellular glycogen content. In line with the observed metabolic heterogeneity, the individual HCCs differed largely in their vulnerability against pharmacological treatment with metformin. Taken together, our approach provided a comprehensive and quantitative characterization of HCC metabolism that may pave the way for a computational a priori assessment of pharmacological therapies targeting metabolic processes of HCC. |
Databáze: | OpenAIRE |
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