Up-Regulation of TAB3 Is Involved in Neuronal Apoptosis After Intracerebral Hemorrhage
| Autor: | Maohong Cao, Kaifu Ke, Rongrong Chen, Xiaojin Ning, Aihua Dai, Xiaorong Liu, Yaohui Ni, Lijian Han, Liang Zhu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Transcriptional Activation Pathology medicine.medical_specialty Central nervous system Apoptosis Nerve Tissue Proteins Biology PC12 Cells 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Downregulation and upregulation Western blot medicine Animals Cells Cultured Cerebral Hemorrhage Intracerebral hemorrhage Neurons medicine.diagnostic_test Kinase Caspase 3 Cell Biology General Medicine medicine.disease Rats Up-Regulation 030104 developmental biology medicine.anatomical_structure Cancer research RNA Interference Neuron Carrier Proteins 030217 neurology & neurosurgery Transforming growth factor |
| Zdroj: | Cellular and molecular neurobiology. 37(4) |
| ISSN: | 1573-6830 |
| Popis: | Human transforming growth factor β-activated kinase (TAK1)-binding protein 3 (TAB3) is a regulator of NF-κB which has been mainly found in a variety of cancers. While TAB3 is highly expressed in brain tissue, little is known about the function of TAB3 in central nervous system. Our group established an animal ICH model with autologous whole blood injected into brain, and also a cell ICH model with hemin stimulation. Our Western blot result showed up-regulation of TAB3 during neuronal apoptosis in the model of intracerebral hemorrhage (ICH), which was also approved by immunofluorescence and immunohistochemistry result. Besides, increasing TAB3 level was accompanied by the increased expression of active-caspase-3, active-caspase-8, and decreased expression of Bcl-2. Furthermore, in in vitro study, the level of neuronal apoptosis was decreased by applying TAB3- RNA interference in PC12 cells. All the results above suggested that TAB3 probably participates in the process of neuronal apoptosis following ICH. |
| Databáze: | OpenAIRE |
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